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https://doi.org/10.1099/vir.0.049635-0
Title: | Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms | Authors: | Gaunt, E.R Zhang, Q Cheung, W Wakelam, M.J.O Lever, A.M.L Desselberger, U |
Keywords: | double stranded RNA fat droplet iodixanol lipidome virus protein animal cell article cell lysate cell organelle controlled study host cell lipid analysis mass spectrometry nonhuman priority journal Rotavirus Rotavirus infection ultracentrifugation virus cell interaction virus genome Animals Cell Line Humans Inclusion Bodies, Viral Kidney Lipids Mass Spectrometry Organelles Rotavirus Ultracentrifugation Virus Replication Rotavirus |
Issue Date: | 2013 | Citation: | Gaunt, E.R, Zhang, Q, Cheung, W, Wakelam, M.J.O, Lever, A.M.L, Desselberger, U (2013). Lipidome analysis of rotavirus-infected cells confirms the close interaction of lipid droplets with viroplasms. Journal of General Virology 94 (PART7) : 1576-1586. ScholarBank@NUS Repository. https://doi.org/10.1099/vir.0.049635-0 | Rights: | Attribution 4.0 International | Abstract: | Rotaviruses (RVs) cause acute gastroenteritis in infants and young children, and are globally distributed. Within the infected host cell, RVs establish replication complexes in viroplasms ('viral factories') to which lipid droplet organelles are recruited. To further understand this recently discovered phenomenon, the lipidomes of RV-infected and uninfected MA104 cells were investigated. Cell lysates were subjected to equilibrium ultracentrifugation through iodixanol gradients. Fourteen different classes of lipids were differentiated by mass spectrometry. The concentrations of virtually all lipids were elevated in RV-infected cells. Fractions of low density (1.11-1.15 g ml-1), in which peaks of the RV dsRNA genome and lipid droplet- and viroplasmassociated proteins were observed, contained increased amounts of lipids typically found concentrated in the cellular organelle lipid droplets, confirming the close interaction of lipid droplets with viroplasms. A decrease in the ratio of the amounts of surface to internal components of lipid droplets upon RV infection suggested that the lipid droplet-viroplasm complexes became enlarged. © 2013 SGM. | Source Title: | Journal of General Virology | URI: | https://scholarbank.nus.edu.sg/handle/10635/180792 | ISSN: | 0022-1317 | DOI: | 10.1099/vir.0.049635-0 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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