Please use this identifier to cite or link to this item: https://doi.org/10.1158/1078-0432.CCR-12-3753
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dc.titleConsistent and differential genetic aberrations between esophageal dysplasia and squamous cell carcinoma detected by array comparative genomic hybridization
dc.contributor.authorShi, Z.-Z
dc.contributor.authorShang, L
dc.contributor.authorJiang, Y.-Y
dc.contributor.authorHao, J.-J
dc.contributor.authorZhang, Y
dc.contributor.authorZhang, T.-T
dc.contributor.authorLin, D.-C
dc.contributor.authorLiu, S.-G
dc.contributor.authorWang, B.-S
dc.contributor.authorGong, T
dc.contributor.authorZhan, Q.-M
dc.contributor.authorWang, M.-R
dc.date.accessioned2020-10-27T04:43:11Z
dc.date.available2020-10-27T04:43:11Z
dc.date.issued2013
dc.identifier.citationShi, Z.-Z, Shang, L, Jiang, Y.-Y, Hao, J.-J, Zhang, Y, Zhang, T.-T, Lin, D.-C, Liu, S.-G, Wang, B.-S, Gong, T, Zhan, Q.-M, Wang, M.-R (2013). Consistent and differential genetic aberrations between esophageal dysplasia and squamous cell carcinoma detected by array comparative genomic hybridization. Clinical Cancer Research 19 (21) : 5867-5878. ScholarBank@NUS Repository. https://doi.org/10.1158/1078-0432.CCR-12-3753
dc.identifier.issn1078-0432
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/180777
dc.description.abstractPurpose: Our aim was to identify frequent genomic aberrations in both esophageal squamous cell carcinoma (ESCC) and esophageal dysplasia and to discover important copy number-driving genes and microRNAs (miRNA) in ESCC. Experimental Design: We conducted array-based comparative genomic hybridization (array CGH) on 59 ESCCresection samples and 16 dysplasia biopsy samples. Expression of genes at 11q13.3 was analyzed by real-time PCR (RT-PCR) and immunohistochemistry (IHC). Integrated analysis was conducted to identify genes or miRNAs with copy number-expression correlations. Results: Array CGH identified 11 amplifications and eight homozygous deletions in ESCC. Integrated analysis of array CGH data with matched gene expression microarray data showed that 90 overexpressed genes and 24 underexpressed genes were consistent with DNA copy number changes, including 12 copy number-driving miRNAs. In esophageal dysplasia, six gains, four losses, 12 amplifications, and four homozygous deletions were detected. Amplifications of 7p11.2 and 11q13.2-11q13.3 (CCND1) and homozygous deletion at 9p21.3 (CDKN2A) were consistent genomic changes in both dysplasia and carcinoma. ANO1 at 11q13.3 was overexpressed at the mRNA and protein levels in tumors, and higher mRNA expression was correlated with the copy number increase. In particular, ANO1 expression was elevated in moderate dysplasia compared with normal esophageal epithelium. IHC revealed that ANO1 overexpression was positively correlated with lymph node metastasis and advanced clinical stage. Knockdown of ANO1 significantly inhibited the proliferation of KYSE30 and KYSE510 cells. Conclusion: Copy number aberrations in both esophageal dysplasia and ESCC may be useful as potential biomarkers for early detection. In addition, ANO1 may be a candidate target gene in esophageal tumorigenesis. © 2013 American Association for Cancer Research.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectcyclin dependent kinase inhibitor 2A
dc.subjectmicroRNA
dc.subjectadvanced cancer
dc.subjectANO1 gene
dc.subjectarticle
dc.subjectcancer staging
dc.subjectCCND1 gene
dc.subjectcdkn2a gene
dc.subjectcell proliferation
dc.subjectcell strain
dc.subjectcell strain KYSE30
dc.subjectcell strain KYSE510
dc.subjectchromosome 11q
dc.subjectchromosome 7p
dc.subjectcomparative genomic hybridization
dc.subjectcontrolled study
dc.subjectesophageal squamous cell carcinoma
dc.subjectesophagus biopsy
dc.subjectesophagus dysplasia
dc.subjectesophagus resection
dc.subjectgene
dc.subjectgene amplification
dc.subjectgene deletion
dc.subjectgene dosage
dc.subjectgene identification
dc.subjectgene loss
dc.subjectgene overexpression
dc.subjectgenetic disorder
dc.subjecthistopathology
dc.subjecthomozygote
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectlymph node metastasis
dc.subjectmajor clinical study
dc.subjectmicroarray analysis
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjectRNA analysis
dc.subjectCarcinoma, Squamous Cell
dc.subjectCell Line, Tumor
dc.subjectChromosome Aberrations
dc.subjectComparative Genomic Hybridization
dc.subjectDNA Copy Number Variations
dc.subjectEsophageal Neoplasms
dc.subjectEsophagus
dc.subjectGene Amplification
dc.subjectGene Deletion
dc.subjectGene Expression
dc.subjectHumans
dc.subjectPrecancerous Conditions
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.description.doi10.1158/1078-0432.CCR-12-3753
dc.description.sourcetitleClinical Cancer Research
dc.description.volume19
dc.description.issue21
dc.description.page5867-5878
dc.published.statePublished
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