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https://doi.org/10.1038/ncomms9054
Title: | Tbx15 controls skeletal muscle fibre-type determination and muscle metabolism | Authors: | Lee, K.Y Singh, M.K Ussar, S Wetzel, P Hirshman, M.F Goodyear, L.J Kispert, A Kahn, C.R |
Keywords: | adenylate kinase somatomedin B Tbx15 protein transcription factor unclassified drug fat intake hydroxymethylglutaryl coenzyme A reductase kinase IGF2 protein, mouse somatomedin B T box transcription factor TBX15 protein, mouse biochemistry bioenergetics gene expression glucose metabolism muscle oxygen physiology skeletal remains animal cell animal experiment animal tissue Article controlled study glucose intolerance glycolysis mouse muscle contraction muscle metabolism muscle relaxation nonhuman obesity oxygen consumption protein expression skeletal muscle administration and dosage adverse effects animal cell line chemically induced classification fat intake gene expression regulation genetics knockout mouse male metabolism oxidation reduction reaction physiology skeletal muscle cell AMP-Activated Protein Kinases Animals Cell Line Dietary Fats Gene Expression Regulation Glucose Intolerance Insulin-Like Growth Factor II Male Mice Mice, Knockout Muscle Contraction Muscle Fibers, Skeletal Obesity Oxidation-Reduction T-Box Domain Proteins |
Issue Date: | 2015 | Publisher: | Nature Publishing Group | Citation: | Lee, K.Y, Singh, M.K, Ussar, S, Wetzel, P, Hirshman, M.F, Goodyear, L.J, Kispert, A, Kahn, C.R (2015). Tbx15 controls skeletal muscle fibre-type determination and muscle metabolism. Nature Communications 6 : 8054. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms9054 | Rights: | Attribution 4.0 International | Abstract: | Skeletal muscle is composed of both slow-twitch oxidative myofibers and fast-twitch glycolytic myofibers that differentially impact muscle metabolism, function and eventually whole-body physiology. Here we show that the mesodermal transcription factor T-box 15 (Tbx15) is highly and specifically expressed in glycolytic myofibers. Ablation of Tbx15 in vivo leads to a decrease in muscle size due to a decrease in the number of glycolytic fibres, associated with a small increase in the number of oxidative fibres. This shift in fibre composition results in muscles with slower myofiber contraction and relaxation, and also decreases whole-body oxygen consumption, reduces spontaneous activity, increases adiposity and glucose intolerance. Mechanistically, ablation of Tbx15 leads to activation of AMPK signalling and a decrease in Igf2 expression. Thus, Tbx15 is one of a limited number of transcription factors to be identified with a critical role in regulating glycolytic fibre identity and muscle metabolism. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/180444 | ISSN: | 2041-1723 | DOI: | 10.1038/ncomms9054 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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