Please use this identifier to cite or link to this item:
https://doi.org/10.1242/jcs.157537
DC Field | Value | |
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dc.title | Opposing roles for JNK and Aurora A in regulating the association of WDR62 with spindle microtubules | |
dc.contributor.author | Lim, N.R | |
dc.contributor.author | Yeap, Y.Y.C | |
dc.contributor.author | Zhao, T.T | |
dc.contributor.author | Yip, Y.Y | |
dc.contributor.author | Wong, S.C | |
dc.contributor.author | Xu, D | |
dc.contributor.author | Ang, C.-S | |
dc.contributor.author | Williamson, N.A | |
dc.contributor.author | Xu, Z | |
dc.contributor.author | Bogoyevitch, M.A | |
dc.contributor.author | Ng, D.C.H | |
dc.date.accessioned | 2020-10-26T06:53:50Z | |
dc.date.available | 2020-10-26T06:53:50Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Lim, N.R, Yeap, Y.Y.C, Zhao, T.T, Yip, Y.Y, Wong, S.C, Xu, D, Ang, C.-S, Williamson, N.A, Xu, Z, Bogoyevitch, M.A, Ng, D.C.H (2015). Opposing roles for JNK and Aurora A in regulating the association of WDR62 with spindle microtubules. Journal of Cell Science 128 (3) : 527-540. ScholarBank@NUS Repository. https://doi.org/10.1242/jcs.157537 | |
dc.identifier.issn | 0021-9533 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/180092 | |
dc.description.abstract | WD40-repeat protein 62 (WDR62) is a spindle pole protein required for normal cell division and neuroprogenitor differentiation during brain development. Microcephaly-associated mutations in WDR62 lead to mitotic mislocalization, highlighting a crucial requirement for precise WDR62 spatiotemporal distribution, although the regulatory mechanisms are unknown. Here, we demonstrate that the WD40-repeat region of WDR62 is required for microtubule association, whereas the disordered C-terminal region regulates cell-cycledependent compartmentalization. In agreement with a functional requirement for the WDR62-JNK1 complex during neurogenesis, WDR62 specifically recruits JNK1 (also known as MAPK8), but not JNK2 (also known as MAPK9), to the spindle pole. However, JNKmediated phosphorylation of WDR62 T1053 negatively regulated microtubule association, and loss of JNK signaling resulted in constitutive WDR62 localization to microtubules irrespective of cell cycle stage. In contrast, we identified that Aurora A kinase (AURKA) and WDR62 were in complex and that AURKA-mediated phosphorylation was required for the spindle localization of WDR62 during mitosis. Our studies highlight complex regulation of WDR62 localization, with opposing roles for JNK and AURKA in determining its spindle association. © 2015. Published by The Company of Biologists Ltd. | |
dc.publisher | Company of Biologists Ltd | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | aurora A kinase | |
dc.subject | mitogen activated protein kinase 9 | |
dc.subject | protein | |
dc.subject | stress activated protein kinase | |
dc.subject | stress activated protein kinase 1 | |
dc.subject | unclassified drug | |
dc.subject | WDR62 protein | |
dc.subject | AURKA protein, human | |
dc.subject | aurora A kinase | |
dc.subject | nerve protein | |
dc.subject | stress activated protein kinase 1 | |
dc.subject | WDR62 protein, human | |
dc.subject | Article | |
dc.subject | cell compartmentalization | |
dc.subject | cell cycle | |
dc.subject | controlled study | |
dc.subject | female | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | microtubule | |
dc.subject | mitosis | |
dc.subject | nervous system development | |
dc.subject | phosphorylation | |
dc.subject | priority journal | |
dc.subject | protein phosphorylation | |
dc.subject | spindle microtubule | |
dc.subject | spindle pole | |
dc.subject | cell line | |
dc.subject | genetics | |
dc.subject | HeLa cell line | |
dc.subject | metabolism | |
dc.subject | microtubule | |
dc.subject | protein tertiary structure | |
dc.subject | signal transduction | |
dc.subject | spindle apparatus | |
dc.subject | Aurora Kinase A | |
dc.subject | Cell Line | |
dc.subject | HeLa Cells | |
dc.subject | Humans | |
dc.subject | MAP Kinase Signaling System | |
dc.subject | Microtubules | |
dc.subject | Mitogen-Activated Protein Kinase 8 | |
dc.subject | Mitosis | |
dc.subject | Nerve Tissue Proteins | |
dc.subject | Neurogenesis | |
dc.subject | Phosphorylation | |
dc.subject | Protein Structure, Tertiary | |
dc.subject | Spindle Apparatus | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1242/jcs.157537 | |
dc.description.sourcetitle | Journal of Cell Science | |
dc.description.volume | 128 | |
dc.description.issue | 3 | |
dc.description.page | 527-540 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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