Please use this identifier to cite or link to this item: https://doi.org/10.1101/gr.190470.115
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dc.titleFrequent somatic transfer of mitochondrial DNA into the nuclear genome of human cancer cells
dc.contributor.authorJu, Y.S
dc.contributor.authorTubio, J.M.C
dc.contributor.authorMifsud, W
dc.date.accessioned2020-10-23T08:13:27Z
dc.date.available2020-10-23T08:13:27Z
dc.date.issued2015
dc.identifier.citationJu, Y.S, Tubio, J.M.C, Mifsud, W (2015). Frequent somatic transfer of mitochondrial DNA into the nuclear genome of human cancer cells. Genome Research 25 (6) : 814-824. ScholarBank@NUS Repository. https://doi.org/10.1101/gr.190470.115
dc.identifier.issn1088-9051
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/179660
dc.description.abstractMitochondrial genomes are separated from the nuclear genome for most of the cell cycle by the nuclear double membrane, intervening cytoplasm, and the mitochondrial double membrane. Despite these physical barriers, we show that somatically acquired mitochondrial-nuclear genome fusion sequences are present in cancer cells. Most occur in conjunction with intranuclear genomic rearrangements, and the features of the fusion fragments indicate that nonhomologous end joining and/or replication-dependent DNA double-strand break repair are the dominant mechanisms involved. Remarkably, mitochondrial-nuclear genome fusions occur at a similar rate per base pair of DNA as interchromosomal nuclear rearrangements, indicating the presence of a high frequency of contact between mitochondrial and nuclear DNA in some somatic cells. Transmission of mitochondrial DNA to the nuclear genome occurs in neoplastically transformed cells, but we do not exclude the possibility that some mitochondrial-nuclear DNA fusions observed in cancer occurred years earlier in normal somatic cells. © 2015 Ju et al.
dc.publisherCold Spring Harbor Laboratory Press
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectmitochondrial DNA
dc.subjectmitochondrial DNA
dc.subjectArticle
dc.subjectbacterial artificial chromosome
dc.subjectcancer cell
dc.subjectcell nucleus transplantation
dc.subjectchromosome 10
dc.subjectchromosome 11
dc.subjectcontrolled study
dc.subjectDNA end joining repair
dc.subjectDNA replication
dc.subjectDNA replication origin
dc.subjectdouble stranded DNA break
dc.subjectfluorescence in situ hybridization
dc.subjectfusion gene
dc.subjectgene expression
dc.subjectgene rearrangement
dc.subjectgene sequence
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman genome
dc.subjectinterphase
dc.subjectmitochondrial DNA replication
dc.subjectmitochondrial genome
dc.subjectprimary tumor
dc.subjectpriority journal
dc.subjectsomatic cell
dc.subjecttransformed cell
dc.subjecttransposon
dc.subjectamino acid sequence
dc.subjectcell nucleus
dc.subjectchromosome
dc.subjectcopy number variation
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjectHeLa cell line
dc.subjectmitochondrion
dc.subjectmolecular genetics
dc.subjectneoplasm
dc.subjectreproducibility
dc.subjecttumor cell line
dc.subjectAmino Acid Sequence
dc.subjectCell Line, Tumor
dc.subjectCell Nucleus
dc.subjectChromosomes
dc.subjectDNA Copy Number Variations
dc.subjectDNA End-Joining Repair
dc.subjectDNA Replication
dc.subjectDNA, Mitochondrial
dc.subjectGenome, Human
dc.subjectGenome, Mitochondrial
dc.subjectHeLa Cells
dc.subjectHumans
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMitochondria
dc.subjectMolecular Sequence Data
dc.subjectNeoplasms
dc.subjectReproducibility of Results
dc.subjectSequence Analysis, DNA
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1101/gr.190470.115
dc.description.sourcetitleGenome Research
dc.description.volume25
dc.description.issue6
dc.description.page814-824
dc.published.statePublished
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