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https://doi.org/10.1530/JME-17-0214
Title: | Curcumin exerts a protective effect against premature ovarian failure in mice | Authors: | Yan, Z Dai, Y Fu, H Zheng, Y Bao, D Yin, Y Chen, Q Nie, X Hao, Q Hou, D Cui, Y |
Keywords: | caspase 3 caspase 9 curcumin galactose malonaldehyde messenger RNA protein kinase B protein p16 superoxide dismutase 3-nitrotyrosine 4-hydroxy-2-nonenal 8-oxo-7-hydrodeoxyguanosine aldehyde curcumin cyclin dependent kinase inhibitor 2A deoxyguanosine messenger RNA Muellerian inhibiting factor protective agent tyrosine animal cell animal experiment animal model animal tissue antral follicle apoptosis Article controlled study developmental stage enzyme immunoassay female granulosa cell immunohistochemistry mouse mRNA expression level nonhuman ovary function ovary tissue oxidative stress premature ovarian failure primordial follicle priority journal protein expression level TUNEL assay Western blotting animal C57BL mouse disease model drug effect genetics gonad hypothalamus hypophysis system metabolism ovary pathology premature ovarian failure Aldehydes Animals Anti-Mullerian Hormone Apoptosis Curcumin Cyclin-Dependent Kinase Inhibitor p16 Deoxyguanosine Disease Models, Animal Female Galactose Gonads Hypothalamo-Hypophyseal System Mice, Inbred C57BL Ovary Oxidative Stress Primary Ovarian Insufficiency Protective Agents RNA, Messenger Tyrosine |
Issue Date: | 2018 | Publisher: | BioScientifica Ltd. | Citation: | Yan, Z, Dai, Y, Fu, H, Zheng, Y, Bao, D, Yin, Y, Chen, Q, Nie, X, Hao, Q, Hou, D, Cui, Y (2018). Curcumin exerts a protective effect against premature ovarian failure in mice. Journal of Molecular Endocrinology 60 (3) : 261-271. ScholarBank@NUS Repository. https://doi.org/10.1530/JME-17-0214 | Rights: | Attribution 4.0 International | Abstract: | This study was designed to investigate the protective effect of curcumin against d-galactose (d-gal)-induced premature ovarian failure (POF) in mice. A mouse POF model was induced by subcutaneous injection of d-gal (200 mg/kg/day) daily for 42 days. Mice in the curcumin group received both d-gal treatment and intraperitoneal injection of curcumin (100 mg/kg/day) for 42 days. Ovarian function, oxidative stress and apoptosis were evaluated. The P, E2 and SOD levels were higher, and the FSH, LH and MDA levels were significantly lower in the curcumin group than those in the d-gal group. The proportion of primordial follicles was also significantly higher in the curcumin group than that in the d-gal group. In addition, curcumin treatment after d-gal administration resulted in significantly lower Sod2, Cat, 8-OhdG, 4-HNE, NTY and senescence-associated protein P16 expression levels, higher Amh expression levels and less apoptosis in granulosa cells than was observed in the d-gal group. Moreover, the p-Akt, Nrf2 and HO-1 protein expression levels were significantly higher and the apoptosis-related cleaved caspase-3 and -9 protein expression levels were markedly lower in the curcumin group than in the d-gal group. In conclusion, curcumin effectively inhibited d-gal-induced oxidative stress, apoptosis and ovarian injury via a mechanism involving the Nrf2/HO-1 and PI3K/Akt signaling pathways, suggesting that curcumin is a potential protective agent against POF. © 2018 Society for Endocrinology Published by Bioscientifica Ltd. | Source Title: | Journal of Molecular Endocrinology | URI: | https://scholarbank.nus.edu.sg/handle/10635/179044 | ISSN: | 09525041 | DOI: | 10.1530/JME-17-0214 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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