Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-017-06233-9
DC Field | Value | |
---|---|---|
dc.title | Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium | |
dc.contributor.author | Hongisto, H | |
dc.contributor.author | Jylhä, A | |
dc.contributor.author | Nättinen, J | |
dc.contributor.author | Rieck, J | |
dc.contributor.author | Ilmarinen, T | |
dc.contributor.author | Veréb, Z | |
dc.contributor.author | Aapola, U | |
dc.contributor.author | Beuerman, R | |
dc.contributor.author | Petrovski, G | |
dc.contributor.author | Uusitalo, H | |
dc.contributor.author | Skottman, H | |
dc.date.accessioned | 2020-10-20T10:29:47Z | |
dc.date.available | 2020-10-20T10:29:47Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Hongisto, H, Jylhä, A, Nättinen, J, Rieck, J, Ilmarinen, T, Veréb, Z, Aapola, U, Beuerman, R, Petrovski, G, Uusitalo, H, Skottman, H (2017). Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium. Scientific Reports 7 (1) : 6016. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-06233-9 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178600 | |
dc.description.abstract | Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) provide an unlimited cell source for retinal cell replacement therapies. Clinical trials using hESC-RPE to treat diseases such as age-related macular degeneration (AMD) are currently underway. Human ESC-RPE cells have been thoroughly characterized at the gene level but their protein expression profile has not been studied at larger scale. In this study, proteomic analysis was used to compare hESC-RPE cells differentiated from two independent hESC lines, to primary human RPE (hRPE) using Isobaric tags for relative quantitation (iTRAQ). 1041 common proteins were present in both hESC-RPE cells and native hRPE with majority of the proteins similarly regulated. The hESC-RPE proteome reflected that of normal hRPE with a large number of metabolic, mitochondrial, cytoskeletal, and transport proteins expressed. No signs of increased stress, apoptosis, immune response, proliferation, or retinal degeneration related changes were noted in hESC-RPE, while important RPE specific proteins involved in key RPE functions such as visual cycle and phagocytosis, could be detected in the hESC-RPE. Overall, the results indicated that the proteome of the hESC-RPE cells closely resembled that of their native counterparts. © 2017 The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | proteome | |
dc.subject | biology | |
dc.subject | cell differentiation | |
dc.subject | gene ontology | |
dc.subject | genetics | |
dc.subject | human | |
dc.subject | human embryonic stem cell | |
dc.subject | mass spectrometry | |
dc.subject | metabolism | |
dc.subject | procedures | |
dc.subject | proteomics | |
dc.subject | retinal pigment epithelium | |
dc.subject | Cell Differentiation | |
dc.subject | Computational Biology | |
dc.subject | Gene Ontology | |
dc.subject | Human Embryonic Stem Cells | |
dc.subject | Humans | |
dc.subject | Mass Spectrometry | |
dc.subject | Proteome | |
dc.subject | Proteomics | |
dc.subject | Retinal Pigment Epithelium | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1038/s41598-017-06233-9 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 7 | |
dc.description.issue | 1 | |
dc.description.page | 6016 | |
dc.published.state | published | |
Appears in Collections: | Staff Publications Elements |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_s41598-017-06233-9.pdf | 2.96 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License