Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-12984-2
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dc.titleMicroscale Bioreactors for in situ characterization of GI epithelial cell physiology
dc.contributor.authorCostello, C.M
dc.contributor.authorPhillipsen, M.B
dc.contributor.authorHartmanis, L.M
dc.contributor.authorKwasnica, M.A
dc.contributor.authorChen, V
dc.contributor.authorHackam, D
dc.contributor.authorChang, M.W
dc.contributor.authorBentley, W.E
dc.contributor.authorMarch, J.C
dc.date.accessioned2020-10-20T10:22:13Z
dc.date.available2020-10-20T10:22:13Z
dc.date.issued2017
dc.identifier.citationCostello, C.M, Phillipsen, M.B, Hartmanis, L.M, Kwasnica, M.A, Chen, V, Hackam, D, Chang, M.W, Bentley, W.E, March, J.C (2017). Microscale Bioreactors for in situ characterization of GI epithelial cell physiology. Scientific Reports 7 (1) : 12515. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-12984-2
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178571
dc.description.abstractThe development of in vitro artificial small intestines that realistically mimic in vivo systems will enable vast improvement of our understanding of the human gut and its impact on human health. Synthetic in vitro models can control specific parameters, including (but not limited to) cell types, fluid flow, nutrient profiles and gaseous exchange. They are also "open" systems, enabling access to chemical and physiological information. In this work, we demonstrate the importance of gut surface topography and fluid flow dynamics which are shown to impact epithelial cell growth, proliferation and intestinal cell function. We have constructed a small intestinal bioreactor using 3-D printing and polymeric scaffolds that mimic the 3-D topography of the intestine and its fluid flow. Our results indicate that TEER measurements, which are typically high in static 2-D Transwell apparatuses, is lower in the presence of liquid sheer and 3-D topography compared to a flat scaffold and static conditions. There was also increased cell proliferation and discovered localized regions of elevated apoptosis, specifically at the tips of the villi, where there is highest sheer. Similarly, glucose was actively transported (as opposed to passive) and at higher rates under flow. © 2017 The Author(s).
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectartificial organ
dc.subjectbiomimetics
dc.subjectbioreactor
dc.subjectCaco-2 cell line
dc.subjectcell proliferation
dc.subjectchemistry
dc.subjectepithelium cell
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjecthuman
dc.subjectintestine mucosa
dc.subjectsmall intestine
dc.subjectthree dimensional printing
dc.subjecttissue engineering
dc.subjecttissue scaffold
dc.subjecttrends
dc.subjectArtificial Organs
dc.subjectBiomimetics
dc.subjectBioreactors
dc.subjectCaco-2 Cells
dc.subjectCell Proliferation
dc.subjectEpithelial Cells
dc.subjectHumans
dc.subjectIntestinal Mucosa
dc.subjectIntestine, Small
dc.subjectPrinting, Three-Dimensional
dc.subjectTissue Engineering
dc.subjectTissue Scaffolds
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1038/s41598-017-12984-2
dc.description.sourcetitleScientific Reports
dc.description.volume7
dc.description.issue1
dc.description.page12515
dc.published.statepublished
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