Please use this identifier to cite or link to this item:
https://doi.org/10.1186/1743-422X-2-24
DC Field | Value | |
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dc.title | Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure | |
dc.contributor.author | Rahman, M | |
dc.contributor.author | Sultana, R | |
dc.contributor.author | Podder, G | |
dc.contributor.author | Faruque, A.S.G | |
dc.contributor.author | Matthijnssens, J | |
dc.contributor.author | Zaman, K | |
dc.contributor.author | Breiman, R.F | |
dc.contributor.author | Sack, D.A | |
dc.contributor.author | Van Ranst, M | |
dc.contributor.author | Azim, T | |
dc.date.accessioned | 2020-10-20T09:33:51Z | |
dc.date.available | 2020-10-20T09:33:51Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Rahman, M, Sultana, R, Podder, G, Faruque, A.S.G, Matthijnssens, J, Zaman, K, Breiman, R.F, Sack, D.A, Van Ranst, M, Azim, T (2005). Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure. Virology Journal 2 : 24. ScholarBank@NUS Repository. https://doi.org/10.1186/1743-422X-2-24 | |
dc.identifier.issn | 1743-422X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178368 | |
dc.description.abstract | Background: Rotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation, different G-type-specific primer sets are applied in different geographical locations. In Bangladesh, a significant proportion (36.9%) of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed. Results: Four nucleotide substitutions at the G1 primer-binding site of the VP7 gene of Bangladeshi G1 rotaviruses rendered a major proportion of circulating strains untypeable using the routine primer set. Using an alternative primer set, we could identify G1 rotaviruses as the most prevalent genotype (44.8%), followed by G9 (21.7%), G2 (15.0%) and G4 (13.8%). Conclusion: Because of the natural variation in the rotaviral gene sequences, close monitoring of rotavirus genotyping methods is important. © 2005 Rahman et al; licensee BioMed Central Ltd. | |
dc.publisher | BMC | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | glycoprotein | |
dc.subject | glycoprotein vp7 | |
dc.subject | unclassified drug | |
dc.subject | amino acid substitution | |
dc.subject | article | |
dc.subject | base mispairing | |
dc.subject | binding site | |
dc.subject | gene sequence | |
dc.subject | genotype | |
dc.subject | Human rotavirus | |
dc.subject | nonhuman | |
dc.subject | nucleotide sequence | |
dc.subject | open reading frame | |
dc.subject | sequence analysis | |
dc.subject | virus strain | |
dc.subject | virus typing | |
dc.subject | Antigens, Viral | |
dc.subject | Base Sequence | |
dc.subject | Capsid Proteins | |
dc.subject | DNA Primers | |
dc.subject | Genetic Variation | |
dc.subject | Genotype | |
dc.subject | Humans | |
dc.subject | Molecular Sequence Data | |
dc.subject | Rotavirus | |
dc.subject | Rotavirus | |
dc.type | Article | |
dc.contributor.department | MECHANICAL ENGINEERING | |
dc.description.doi | 10.1186/1743-422X-2-24 | |
dc.description.sourcetitle | Virology Journal | |
dc.description.volume | 2 | |
dc.description.page | 24 | |
dc.published.state | published | |
Appears in Collections: | Staff Publications Elements |
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