Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-018-31197-9
DC Field | Value | |
---|---|---|
dc.title | A novel molecular rotor facilitates detection of p53-DNA interactions using the Fluorescent Intercalator Displacement Assay | |
dc.contributor.author | Goh, W.L | |
dc.contributor.author | Lee, M.Y | |
dc.contributor.author | Lim, T.X | |
dc.contributor.author | Chua, J.S | |
dc.contributor.author | Brenner, S | |
dc.contributor.author | Ghadessy, F.J | |
dc.contributor.author | Teo, Y.N | |
dc.date.accessioned | 2020-10-20T03:25:22Z | |
dc.date.available | 2020-10-20T03:25:22Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Goh, W.L, Lee, M.Y, Lim, T.X, Chua, J.S, Brenner, S, Ghadessy, F.J, Teo, Y.N (2018). A novel molecular rotor facilitates detection of p53-DNA interactions using the Fluorescent Intercalator Displacement Assay. Scientific Reports 8 (1) : 12946. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-31197-9 | |
dc.identifier.issn | 20452322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/177810 | |
dc.description.abstract | We have investigated the use of fluorescent molecular rotors as probes for detection of p53 binding to DNA. These are a class of fluorophores that undergo twisted intramolecular charge transfer (TICT). They are non-fluorescent in a freely rotating conformation and experience a fluorescence increase when restricted in the planar conformation. We hypothesized that intercalation of a molecular rotor between DNA base pairs would result in a fluorescence turn-on signal. Upon displacement by a DNA binding protein, measurable loss of signal would facilitate use of the molecular rotor in the fluorescent intercalator displacement (FID) assay. A panel of probes was interrogated using the well-established p53 model system across various DNA response elements. A novel, readily synthesizable molecular rotor incorporating an acridine orange DNA intercalating group (AO-R) outperformed other conventional dyes in the FID assay. It enabled relative measurement of p53 sequence-specific DNA interactions and study of the dominant-negative effects of cancer-associated p53 mutants. In a further application, AO-R also proved useful for staining apoptotic cells in live zebrafish embryos. © 2018, The Author(s). | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | DNA | |
dc.subject | fluorescent dye | |
dc.subject | intercalating agent | |
dc.subject | protein p53 | |
dc.subject | TP53 protein, human | |
dc.subject | chemistry | |
dc.subject | human | |
dc.subject | metabolism | |
dc.subject | spectrofluorometry | |
dc.subject | DNA | |
dc.subject | Fluorescent Dyes | |
dc.subject | Humans | |
dc.subject | Intercalating Agents | |
dc.subject | Spectrometry, Fluorescence | |
dc.subject | Tumor Suppressor Protein p53 | |
dc.type | Article | |
dc.contributor.department | MEDICINE | |
dc.description.doi | 10.1038/s41598-018-31197-9 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 8 | |
dc.description.issue | 1 | |
dc.description.page | 12946 | |
Appears in Collections: | Staff Publications Elements |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_s41598-018-31197-9.pdf | 3.62 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License