Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms161226119
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dc.titleInduced pluripotency and gene editing in disease modelling: Perspectives and challenges
dc.contributor.authorSeah, Y.F.S
dc.contributor.authorEl Farran, C.A
dc.contributor.authorWarrier, T
dc.contributor.authorXu, J
dc.contributor.authorLoh, Y.-H
dc.date.accessioned2020-09-14T08:15:37Z
dc.date.available2020-09-14T08:15:37Z
dc.date.issued2015
dc.identifier.citationSeah, Y.F.S, El Farran, C.A, Warrier, T, Xu, J, Loh, Y.-H (2015). Induced pluripotency and gene editing in disease modelling: Perspectives and challenges. International Journal of Molecular Sciences 16 (12) : 28614-28634. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms161226119
dc.identifier.issn1661-6596
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/176139
dc.description.abstractEmbryonic stem cells (ESCs) are chiefly characterized by their ability to self-renew and to differentiate into any cell type derived from the three main germ layers. It was demonstrated that somatic cells could be reprogrammed to form induced pluripotent stem cells (iPSCs) via various strategies. Gene editing is a technique that can be used to make targeted changes in the genome, and the efficiency of this process has been significantly enhanced by recent advancements. The use of engineered endonucleases, such as homing endonucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and Cas9 of the CRISPR system, has significantly enhanced the efficiency of gene editing. The combination of somatic cell reprogramming with gene editing enables us to model human diseases in vitro, in a manner considered superior to animal disease models. In this review, we discuss the various strategies of reprogramming and gene targeting with an emphasis on the current advancements and challenges of using these techniques to model human diseases. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
dc.sourceUnpaywall 20200831
dc.subjectalkaline phosphatase
dc.subjectalpha 1 antitrypsin
dc.subjectcaspase 9
dc.subjectleucine rich repeat kinase 2
dc.subjectmicroRNA
dc.subjectoctamer transcription factor 4
dc.subjectstage specific embryo antigen 1
dc.subjecttranscription activator like effector nuclease
dc.subjecttranscription factor Sox2
dc.subjecttransforming growth factor beta
dc.subjectzinc finger protein
dc.subjectadrenoleukodystrophy
dc.subjectAlzheimer disease
dc.subjectArticle
dc.subjectclustered regularly interspaced short palindromic repeat
dc.subjectCrigler Najjar syndrome
dc.subjectDNA methylation
dc.subjectembryonic stem cell
dc.subjectfragile X syndrome
dc.subjectgene editing
dc.subjectgene targeting
dc.subjectglycogen storage disease type 1
dc.subjectherpes simplex
dc.subjectherpes zoster
dc.subjecthuman
dc.subjectnuclear reprogramming
dc.subjectpancreas adenocarcinoma
dc.subjectpluripotent stem cell
dc.subjectPrader Willi syndrome
dc.subjectprogeria
dc.subjecttelomere
dc.subjecttransgene
dc.subjectWilliams Beuren syndrome
dc.subjectanimal
dc.subjectbiological model
dc.subjectCRISPR Cas system
dc.subjectcytology
dc.subjectgenetic engineering
dc.subjectinduced pluripotent stem cell
dc.subjectmetabolism
dc.subjectnuclear reprogramming
dc.subjectAnimals
dc.subjectCellular Reprogramming
dc.subjectCRISPR-Cas Systems
dc.subjectGene Targeting
dc.subjectGenetic Engineering
dc.subjectHumans
dc.subjectInduced Pluripotent Stem Cells
dc.subjectModels, Biological
dc.subjectTransgenes
dc.typeArticle
dc.contributor.departmentBIOLOGY (NU)
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.3390/ijms161226119
dc.description.sourcetitleInternational Journal of Molecular Sciences
dc.description.volume16
dc.description.issue12
dc.description.page28614-28634
dc.published.statePublished
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