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https://doi.org/10.1038/srep31068
DC Field | Value | |
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dc.title | Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures | |
dc.contributor.author | Yeo, H.C | |
dc.contributor.author | Ting, S | |
dc.contributor.author | Brena, R.M | |
dc.contributor.author | Koh, G | |
dc.contributor.author | Chen, A | |
dc.contributor.author | Toh, S.Q | |
dc.contributor.author | Lim, Y.M | |
dc.contributor.author | Oh, S.K.W | |
dc.contributor.author | Lee, D.-Y | |
dc.date.accessioned | 2020-09-09T01:28:57Z | |
dc.date.available | 2020-09-09T01:28:57Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Yeo, H.C, Ting, S, Brena, R.M, Koh, G, Chen, A, Toh, S.Q, Lim, Y.M, Oh, S.K.W, Lee, D.-Y (2016). Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures. Scientific Reports 6 : 31068. ScholarBank@NUS Repository. https://doi.org/10.1038/srep31068 | |
dc.identifier.issn | 20452322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/174940 | |
dc.description.abstract | The differentiation efficiency of human embryonic stem cells (hESCs) into heart muscle cells (cardiomyocytes) is highly sensitive to culture conditions. To elucidate the regulatory mechanisms involved, we investigated hESCs grown on three distinct culture platforms: feeder-free Matrigel, mouse embryonic fibroblast feeders, and Matrigel replated on feeders. At the outset, we profiled and quantified their differentiation efficiency, transcriptome, transcription factor binding sites and DNA-methylation. Subsequent genome-wide analyses allowed us to reconstruct the relevant interactome, thereby forming the regulatory basis for implicating the contrasting differentiation efficiency of the culture conditions. We hypothesized that the parental expressions of FOXC1, FOXD1 and FOXQ1 transcription factors (TFs) are correlative with eventual cardiomyogenic outcome. Through WNT induction of the FOX TFs, we observed the co-activation of WNT3 and EOMES which are potent inducers of mesoderm differentiation. The result strengthened our hypothesis on the regulatory role of the FOX TFs in enhancing mesoderm differentiation capacity of hESCs. Importantly, the final proportions of cells expressing cardiac markers were directly correlated to the strength of FOX inductions within 72 hours after initiation of differentiation across different cell lines and protocols. Thus, we affirmed the relationship between early FOX TF expressions and cardiomyogenesis efficiency. © The Author(s) 2016. | |
dc.publisher | Nature Publishing Group | |
dc.source | Unpaywall 20200831 | |
dc.subject | collagen | |
dc.subject | forkhead transcription factor | |
dc.subject | laminin | |
dc.subject | matrigel | |
dc.subject | proteoglycan | |
dc.subject | Wnt protein | |
dc.subject | animal | |
dc.subject | binding site | |
dc.subject | biological model | |
dc.subject | cardiac muscle cell | |
dc.subject | cell culture | |
dc.subject | cell culture technique | |
dc.subject | cell differentiation | |
dc.subject | cytology | |
dc.subject | drug combination | |
dc.subject | feeder cell | |
dc.subject | gene expression profiling | |
dc.subject | genetic epigenesis | |
dc.subject | genetics | |
dc.subject | genome-wide association study | |
dc.subject | human | |
dc.subject | human embryonic stem cell | |
dc.subject | mesoderm | |
dc.subject | metabolism | |
dc.subject | mouse | |
dc.subject | physiology | |
dc.subject | procedures | |
dc.subject | signal transduction | |
dc.subject | Animals | |
dc.subject | Binding Sites | |
dc.subject | Cell Culture Techniques | |
dc.subject | Cell Differentiation | |
dc.subject | Cells, Cultured | |
dc.subject | Collagen | |
dc.subject | Drug Combinations | |
dc.subject | Epigenesis, Genetic | |
dc.subject | Feeder Cells | |
dc.subject | Forkhead Transcription Factors | |
dc.subject | Gene Expression Profiling | |
dc.subject | Genome-Wide Association Study | |
dc.subject | Human Embryonic Stem Cells | |
dc.subject | Humans | |
dc.subject | Laminin | |
dc.subject | Mesoderm | |
dc.subject | Mice | |
dc.subject | Models, Cardiovascular | |
dc.subject | Myocytes, Cardiac | |
dc.subject | Proteoglycans | |
dc.subject | Signal Transduction | |
dc.subject | Wnt Proteins | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1038/srep31068 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 6 | |
dc.description.page | 31068 | |
Appears in Collections: | Elements Staff Publications |
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