Please use this identifier to cite or link to this item: https://doi.org/10.3389/fbioe.2018.00069
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dc.titleTemporal changes in nucleus morphology, Lamin A/C and histone methylation during nanotopography-induced neuronal differentiation of stem cells
dc.contributor.authorAnkam S.
dc.contributor.authorTeo B.K.K.
dc.contributor.authorPohan G.
dc.contributor.authorHo S.W.L.
dc.contributor.authorLim C.K.
dc.contributor.authorYim E.K.F.
dc.date.accessioned2020-09-08T03:23:52Z
dc.date.available2020-09-08T03:23:52Z
dc.date.issued2018
dc.identifier.citationAnkam S., Teo B.K.K., Pohan G., Ho S.W.L., Lim C.K., Yim E.K.F. (2018). Temporal changes in nucleus morphology, Lamin A/C and histone methylation during nanotopography-induced neuronal differentiation of stem cells. Frontiers in Bioengineering and Biotechnology 6 (MAY) : 69. ScholarBank@NUS Repository. https://doi.org/10.3389/fbioe.2018.00069
dc.identifier.issn2296-4185
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174602
dc.description.abstractStem cell differentiation can be regulated by biophysical cues such as nanotopography. It involves sensing and integration of these biophysical cues into their transcriptome with a mechanism that is yet to be discovered. In addition to the cytoskeletal and focal adhesion remodeling, nanotopography has also been shown to modulate nucleus morphology. Here, we studied the effect of nanotopography on the temporal changes in nuclei of human embryonic stem cells (hESCs) and human mesenchymal stem cells (hMSCs). Using a high throughput Multi-architecture (MARC) chip analysis, the circularity of the stem cell nuclei changed significantly on different patterns. Human ESCs and MSCs showed different temporal changes in nucleus morphology, lamin A/C expression and histone methylation during topography-induced neuronal differentiation. In hESCs, the expression of nuclear matrix protein, lamin A/C during neuronal differentiation of hESCs on PDMS samples were weakly detected in the first 7 days of differentiation. The histone 3 trimethylation on Lysine 9 (H3K9me3) decreased after differentiation initiated and showed temporal changes in their expression and organization during neuronal differentiation. In hMSCs, the expression of lamin A/C was significantly increased after the first 24 h of cell culture. The quantitative analysis of histone methylation also showed a significant increase in hMSCs histone methylation on 250 nm anisotropic nanogratings within the first 24 h of seeding. This reiterates the importance of cell-substrate sensing within the first 24 h for adult stem cells. The lamin A/C expression and histone methylation shows a correlation of epigenetic changes in early events of differentiation, giving an insight on how extracellular nanotopographical cues are transduced into nuclear biochemical signals. Collectively, these results provide more understanding into the nuclear regulation of the mechanotransduction of nanotopographical cues in stem cell differentiation. © 2018 Ankam, Teo, Pohan, Ho, Lim and Yim.
dc.sourceUnpaywall 20200831
dc.subjectAlkylation
dc.subjectAmino acids
dc.subjectCell culture
dc.subjectMethylation
dc.subjectMorphology
dc.subjectNanotechnology
dc.subjectNeurons
dc.subjectEmbryonic stem cells
dc.subjectEpigenetics
dc.subjectMechanotransduction
dc.subjectMesenchymal stem cell
dc.subjectNeuronal differentiation
dc.subjectNuclear changes
dc.subjectNucleus
dc.subjectStem cells
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.3389/fbioe.2018.00069
dc.description.sourcetitleFrontiers in Bioengineering and Biotechnology
dc.description.volume6
dc.description.issueMAY
dc.description.page69
dc.published.statePublished
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