Please use this identifier to cite or link to this item:
https://doi.org/10.3389/fimmu.2018.02225
DC Field | Value | |
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dc.title | Cross-reactivity and anti-viral function of dengue capsid and NS3- specific memory t cells toward Zika Virus | |
dc.contributor.author | Lim, M.Q. | |
dc.contributor.author | Kumaran, E.A.P. | |
dc.contributor.author | Tan, H.C. | |
dc.contributor.author | Lye, D.C. | |
dc.contributor.author | Leo, Y.S. | |
dc.contributor.author | Ooi, E.E. | |
dc.contributor.author | MacAry, P.A. | |
dc.contributor.author | Bertoletti, A. | |
dc.contributor.author | Rivino, L. | |
dc.date.accessioned | 2020-09-07T05:03:37Z | |
dc.date.available | 2020-09-07T05:03:37Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Lim, M.Q., Kumaran, E.A.P., Tan, H.C., Lye, D.C., Leo, Y.S., Ooi, E.E., MacAry, P.A., Bertoletti, A., Rivino, L. (2018). Cross-reactivity and anti-viral function of dengue capsid and NS3- specific memory t cells toward Zika Virus. Frontiers in Immunology 9 (OCT) : 2225. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2018.02225 | |
dc.identifier.issn | 16643224 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/174526 | |
dc.description.abstract | Zika virus (ZIKV), a flavivirus with homology to dengue virus (DENV), is spreading to areas of DENV hyper-endemicity. Heterologous T cell immunity, whereby virus-specific memory T cells are activated by variant peptides derived from a different virus, can lead to enhanced viral clearance or diminished protective immunity and altered immunopathology. In mice, CD8+ T cells specific for DENV provide in vivo protective efficacy against subsequent ZIKV infection. In humans, contrasting studies report complete absence or varying degrees of DENV/ZIKV T cell cross-reactivity. Moreover, the impact of cross-reactive T cell recognition on the anti-viral capacity of T cells remains unclear. Here, we show that DENV-specific memory T cells display robust cross-reactive recognition of ZIKV NS3 ex vivo and after in vitro expansion in respectively n = 7/10 and n = 9/9 dengue-immune individuals tested. In contrast, cross-reactivity toward ZIKV capsid is low or absent. Cross-reactive recognition of DENV or ZIKV NS3 peptides elicits similar production of the anti-viral effector mediators IFN-γ, TNF-α, and CD107a. We identify 9 DENV/ZIKV cross-reactive epitopes, 7 of which are CD4+ and 2 are CD8+ T cell epitopes. We also show that cross-reactive CD4+ and CD8+ T cells targeting novel NS3 epitopes display anti-viral effector potential toward ZIKV-infected cells, with CD8+ T cells mediating direct lyses of these cells. Our results demonstrate that DENV NS3-specific memory T cells display anti-viral effector capacity toward ZIKV, suggesting a potential beneficial effect in humans of pre-existing T cell immunity to DENV upon ZIKV infection. © 2018 Lim, Kumaran, Tan, Lye, Leo, Ooi, MacAry, Bertoletti and Rivino. | |
dc.publisher | Frontiers Media S.A. | |
dc.source | Unpaywall 20200831 | |
dc.subject | cytokine | |
dc.subject | epitope | |
dc.subject | NS3 protein, flavivirus | |
dc.subject | RNA helicase | |
dc.subject | serine proteinase | |
dc.subject | viral protein | |
dc.subject | blood | |
dc.subject | CD4+ T lymphocyte | |
dc.subject | CD8+ T lymphocyte | |
dc.subject | cell culture | |
dc.subject | cross reaction | |
dc.subject | dengue | |
dc.subject | Dengue virus | |
dc.subject | heterologous immunity | |
dc.subject | human | |
dc.subject | immunological memory | |
dc.subject | immunology | |
dc.subject | virus capsid | |
dc.subject | Zika fever | |
dc.subject | Zika virus | |
dc.subject | Capsid | |
dc.subject | CD4-Positive T-Lymphocytes | |
dc.subject | CD8-Positive T-Lymphocytes | |
dc.subject | Cells, Cultured | |
dc.subject | Cross Reactions | |
dc.subject | Cytokines | |
dc.subject | Dengue | |
dc.subject | Dengue Virus | |
dc.subject | Epitopes, T-Lymphocyte | |
dc.subject | Humans | |
dc.subject | Immunity, Heterologous | |
dc.subject | Immunologic Memory | |
dc.subject | RNA Helicases | |
dc.subject | Serine Endopeptidases | |
dc.subject | Viral Nonstructural Proteins | |
dc.subject | Zika Virus | |
dc.subject | Zika Virus Infection | |
dc.type | Article | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.contributor.department | SAW SWEE HOCK SCHOOL OF PUBLIC HEALTH | |
dc.description.doi | 10.3389/fimmu.2018.02225 | |
dc.description.sourcetitle | Frontiers in Immunology | |
dc.description.volume | 9 | |
dc.description.issue | OCT | |
dc.description.page | 2225 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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