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Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-018-03337-2
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dc.titleA systematic approach to the development of a safe live attenuated Zika vaccine
dc.contributor.authorKwek, S.S
dc.contributor.authorWatanabe, S
dc.contributor.authorChan, K.R
dc.contributor.authorOng, E.Z
dc.contributor.authorTan, H.C
dc.contributor.authorNg, W.C
dc.contributor.authorNguyen, M.T.X
dc.contributor.authorGan, E.S
dc.contributor.authorZhang, S.L
dc.contributor.authorChan, K.W.K
dc.contributor.authorTan, J.H
dc.contributor.authorSessions, O.M
dc.contributor.authorManuel, M
dc.contributor.authorPompon, J
dc.contributor.authorChua, C
dc.contributor.authorHazirah, S
dc.contributor.authorTryggvason, K
dc.contributor.authorVasudevan, S.G
dc.contributor.authorOoi, E.E
dc.date.accessioned2020-09-04T01:51:39Z
dc.date.available2020-09-04T01:51:39Z
dc.date.issued2018
dc.identifier.citationKwek, S.S, Watanabe, S, Chan, K.R, Ong, E.Z, Tan, H.C, Ng, W.C, Nguyen, M.T.X, Gan, E.S, Zhang, S.L, Chan, K.W.K, Tan, J.H, Sessions, O.M, Manuel, M, Pompon, J, Chua, C, Hazirah, S, Tryggvason, K, Vasudevan, S.G, Ooi, E.E (2018). A systematic approach to the development of a safe live attenuated Zika vaccine. Nature Communications 9 (1) : 1031. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03337-2
dc.identifier.issn2041-1723
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174234
dc.description.abstractZika virus (ZIKV) is a flavivirus that can cause congenital disease and requires development of an effective long-term preventative strategy. A replicative ZIKV vaccine with properties similar to the yellow fever 17D (YF17D) live-attenuated vaccine (LAV) would be advantageous, as a single dose of YF17D produces lifelong immunity. However, a replicative ZIKV vaccine must also be safe from causing persistent organ infections. Here we report an approach to ZIKV LAV development. We identify a ZIKV variant that produces small plaques due to interferon (IFN)-restricted viral propagation and displays attenuated infection of endothelial cells. We show that these properties collectively reduce the risk of organ infections and vertical transmission in a mouse model but remain sufficiently immunogenic to prevent wild-type ZIKV infection. Our findings suggest a strategy for the development of a safe but efficacious ZIKV LAV. © 2018 The Author(s).
dc.publisherNature Publishing Group
dc.sourceUnpaywall 20200831
dc.subjectinterferon regulatory factor 3
dc.subjectlive vaccine
dc.subjectZika virus vaccine
dc.subjectlive vaccine
dc.subjectvirus vaccine
dc.subjectcell
dc.subjectimmunity
dc.subjectrodent
dc.subjectstrategic approach
dc.subjectvaccine
dc.subjectvertical transmission
dc.subjectyellow fever
dc.subjectZika virus disease
dc.subjectadult
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectB lymphocyte
dc.subjectcontrolled study
dc.subjectdendritic cell
dc.subjectdrug efficacy
dc.subjectdrug mechanism
dc.subjectdrug safety
dc.subjectembryo
dc.subjectfemale
dc.subjectfetus
dc.subjectgene activation
dc.subjectgenetic variability
dc.subjectgenomic instability
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro propagation
dc.subjectinfection risk
dc.subjectinnate immunity
dc.subjectmale
dc.subjectmouse
dc.subjectmouse model
dc.subjectnext generation sequencing
dc.subjectnonhuman
dc.subjectsingle nucleotide polymorphism
dc.subjectT lymphocyte
dc.subjectumbilical vein endothelial cell
dc.subjectvaccine immunogenicity
dc.subjectVero cell line
dc.subjectvertical transmission
dc.subjectvirus culture
dc.subjectvirus mutation
dc.subjectvirus plaque
dc.subjectvirus transmission
dc.subjectwhole genome sequencing
dc.subjectwild type
dc.subjectZika fever
dc.subjectZika virus
dc.subjectAedes
dc.subjectanimal
dc.subjectevaluation study
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectimmunological technique
dc.subjectimmunology
dc.subjectvirology
dc.subjectZika fever
dc.subjectFlavivirus
dc.subjectHuman immunodeficiency virus 1
dc.subjectZika virus
dc.subjectAedes
dc.subjectAnimals
dc.subjectDendritic Cells
dc.subjectHumans
dc.subjectImmunologic Techniques
dc.subjectMice
dc.subjectVaccines, Attenuated
dc.subjectViral Vaccines
dc.subjectZika Virus
dc.subjectZika Virus Infection
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1038/s41467-018-03337-2
dc.description.sourcetitleNature Communications
dc.description.volume9
dc.description.issue1
dc.description.page1031
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