Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-018-25489-3
DC Field | Value | |
---|---|---|
dc.title | MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells | |
dc.contributor.author | Wang, J | |
dc.contributor.author | Tong, K.S | |
dc.contributor.author | Wong, L.L | |
dc.contributor.author | Liew, O.-W | |
dc.contributor.author | Raghuram, D | |
dc.contributor.author | Richards, A.M | |
dc.contributor.author | Chen, Y.-T | |
dc.date.accessioned | 2020-09-04T01:49:23Z | |
dc.date.available | 2020-09-04T01:49:23Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Wang, J, Tong, K.S, Wong, L.L, Liew, O.-W, Raghuram, D, Richards, A.M, Chen, Y.-T (2018). MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells. Scientific Reports 8 (1) : 7055. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-25489-3 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/174223 | |
dc.description.abstract | Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the expression of NPR3 could be modulated by microRNA-143 (miR-143-3p), a microRNA species with up-regulated circulating concentrations in clinical heart failure. The regulatory effect of miR-143 on NPR3 expression was further evidenced by the reciprocal relationship between miR-143 and NPR3 levels observed in hypoxia-treated human cardiac cells and in left ventricular tissue from rats undergoing experimental myocardial infarction. Further analysis indicated elevation of miR-143 in response to hypoxic challenge reflects transcriptional activation of the miR-143 host gene (MIR143HG). This was corroborated by demonstration of the induction of host gene promoter activity upon hypoxic challenge. Moreover, miR-143 was shown to enhance its own expression by increasing MIR143HG promoter activity, as well as targeting the expressions of NPPA, NPPC, NR3C2, and CRHR2 in cardiac cells. Taken together, these findings suggest that the elevation of miR-143 upon hypoxic insult may be part of a microRNA-based feed forward loop that results in fine tuning the levels of NPs and neurohormonal receptors in cardiac cell lineages. © 2018 The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.source | Unpaywall 20200831 | |
dc.subject | atrial natriuretic factor receptor | |
dc.subject | biological marker | |
dc.subject | microRNA | |
dc.subject | MIRN143 microRNA, human | |
dc.subject | MIRN143 microRNA, rat | |
dc.subject | natriuretic peptide receptor C | |
dc.subject | neuropeptide | |
dc.subject | transcription factor | |
dc.subject | 3' untranslated region | |
dc.subject | animal | |
dc.subject | biological model | |
dc.subject | blood | |
dc.subject | cardiac muscle cell | |
dc.subject | case control study | |
dc.subject | cell line | |
dc.subject | gene expression regulation | |
dc.subject | genetic transcription | |
dc.subject | genetics | |
dc.subject | heart failure | |
dc.subject | human | |
dc.subject | hypoxia | |
dc.subject | metabolism | |
dc.subject | pathophysiology | |
dc.subject | promoter region | |
dc.subject | rat | |
dc.subject | RNA interference | |
dc.subject | 3' Untranslated Regions | |
dc.subject | Animals | |
dc.subject | Biomarkers | |
dc.subject | Case-Control Studies | |
dc.subject | Cell Line | |
dc.subject | Gene Expression Regulation | |
dc.subject | Heart Failure | |
dc.subject | Humans | |
dc.subject | Hypoxia | |
dc.subject | MicroRNAs | |
dc.subject | Models, Biological | |
dc.subject | Myocytes, Cardiac | |
dc.subject | Neuropeptides | |
dc.subject | Promoter Regions, Genetic | |
dc.subject | Rats | |
dc.subject | Receptors, Atrial Natriuretic Factor | |
dc.subject | RNA Interference | |
dc.subject | Transcription Factors | |
dc.subject | Transcription, Genetic | |
dc.type | Article | |
dc.contributor.department | MEDICINE | |
dc.description.doi | 10.1038/s41598-018-25489-3 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 8 | |
dc.description.issue | 1 | |
dc.description.page | 7055 | |
Appears in Collections: | Elements Staff Publications |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_s41598-018-25489-3.pdf | 1.37 MB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.