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https://doi.org/10.1038/srep32036
DC Field | Value | |
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dc.title | Exactin: A specific inhibitor of Factor X activation by extrinsic tenase complex from the venom of Hemachatus haemachatus | |
dc.contributor.author | Girish V.M. | |
dc.contributor.author | Kini R.M.R. | |
dc.date.accessioned | 2020-09-02T06:50:40Z | |
dc.date.available | 2020-09-02T06:50:40Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Girish V.M., Kini R.M.R. (2016). Exactin: A specific inhibitor of Factor X activation by extrinsic tenase complex from the venom of Hemachatus haemachatus. Scientific Reports 6 : 32036. ScholarBank@NUS Repository. https://doi.org/10.1038/srep32036 | |
dc.identifier.issn | 20452322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/173994 | |
dc.description.abstract | Unwanted clots lead to heart attack and stroke that result in a large number of deaths. Currently available anticoagulants have some drawbacks including their non-specific actions. Therefore novel anticoagulants that target specific steps in the coagulation pathway are being sought. Here we describe the identification and characterization of a novel anticoagulant protein from the venom of Hemachatus haemachatus (African Ringhals cobra) that specifically inhibits factor X (FX) activation by the extrinsic tenase complex (ETC) and thus named as exactin. Exactin belongs to the three-finger toxin (3FTx) family, with high sequence identity to neurotoxins and low identity to the well-characterized 3FTx anticoagulants-hemextin and naniproin. It is a mixed-type inhibitor of ETC with the kinetic constants, Ki' and Ki determined as 30.62 ± 7.73 nM and 153.75 ± 17.96 nM, respectively. Exactin does not bind to the active site of factor VIIa and factor Xa based on its weak inhibition (IC 50 ‰ 300 ?M) to the amidolytic activities of these proteases. Exactin shows exquisite macromolecular specificity to FX activation as compared to factor IX activation by ETC. Exactin thus displays a distinct mechanism when compared to other anticoagulants targeting ETC, with its selective preference to ETC-FX [ES] complex. © The Author(s) 2016. | |
dc.source | Unpaywall 20200831 | |
dc.subject | anticoagulant agent | |
dc.subject | blood clotting factor 10 | |
dc.subject | cancer procoagulant | |
dc.subject | cysteine proteinase | |
dc.subject | snake venom | |
dc.subject | tumor protein | |
dc.subject | animal | |
dc.subject | antagonists and inhibitors | |
dc.subject | blood clotting test | |
dc.subject | chemistry | |
dc.subject | circular dichroism | |
dc.subject | Hemachatus | |
dc.subject | human | |
dc.subject | metabolism | |
dc.subject | procedures | |
dc.subject | prothrombin time | |
dc.subject | sequence analysis | |
dc.subject | Animals | |
dc.subject | Anticoagulants | |
dc.subject | Blood Coagulation Tests | |
dc.subject | Circular Dichroism | |
dc.subject | Cysteine Endopeptidases | |
dc.subject | Elapid Venoms | |
dc.subject | Factor X | |
dc.subject | Hemachatus | |
dc.subject | Humans | |
dc.subject | Neoplasm Proteins | |
dc.subject | Prothrombin Time | |
dc.subject | Sequence Analysis, Protein | |
dc.type | Article | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1038/srep32036 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 6 | |
dc.description.page | 32036 | |
Appears in Collections: | Elements Staff Publications |
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