Please use this identifier to cite or link to this item:
https://doi.org/10.3389/fncel.2016.00298
DC Field | Value | |
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dc.title | Neuronal cell bodies remotely regulate axonal growth response to localized netrin-1 treatment via second messenger and DCC dynamics | |
dc.contributor.author | Blasiak, A | |
dc.contributor.author | Kilinc, D | |
dc.contributor.author | Lee, G.U | |
dc.date.accessioned | 2020-09-01T08:02:55Z | |
dc.date.available | 2020-09-01T08:02:55Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Blasiak, A, Kilinc, D, Lee, G.U (2017). Neuronal cell bodies remotely regulate axonal growth response to localized netrin-1 treatment via second messenger and DCC dynamics. Frontiers in Cellular Neuroscience 10 : 298. ScholarBank@NUS Repository. https://doi.org/10.3389/fncel.2016.00298 | |
dc.identifier.issn | 16625102 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/173873 | |
dc.description.abstract | Netrin-1 modulates axonal growth direction and speed. Its best characterized receptor, Deleted in Colorectal Cancer (DCC), is localized to growth cones, but also observed in the cell bodies. We hypothesized that cell bodies sense Netrin-1 and contribute to axon growth rate modulation, mediated by the second messenger system. We cultured mouse cortical neurons in microfluidic devices to isolate distal axon and cell body microenvironments. Compared to isolated axonal treatment, global Netrin-1 treatment decreased the axon elongation rate and affected the dynamics of total and membranous DCC, calcium, and cyclic nucleotides. Signals induced by locally applied Netrin-1 propagated in both anterograde and retrograde directions, demonstrated by the long-range increase in DCC and by the increased frequency of calcium transients in cell bodies, evoked by axonal Netrin-1. Blocking the calcium efflux from endoplasmic reticulum suppressed the membranous DCC response. Our findings support the notion that neurons sense Netrin-1 along their entire lengths in making axonal growth decisions. © 2017 Blasiak, Kilinc and Lee. | |
dc.publisher | Frontiers Media S.A. | |
dc.source | Unpaywall 20200831 | |
dc.subject | carrier proteins and binding proteins | |
dc.subject | cyclic AMP | |
dc.subject | deleted in colorectal cancer | |
dc.subject | netrin 1 | |
dc.subject | ryanodine receptor | |
dc.subject | unclassified drug | |
dc.subject | animal cell | |
dc.subject | Article | |
dc.subject | brain cell culture | |
dc.subject | calcium transport | |
dc.subject | cell elongation | |
dc.subject | controlled study | |
dc.subject | endoplasmic reticulum | |
dc.subject | fluorescence resonance energy transfer | |
dc.subject | genetic transfection | |
dc.subject | growth cone | |
dc.subject | growth rate | |
dc.subject | imaging | |
dc.subject | immunocytochemistry | |
dc.subject | microfluidics | |
dc.subject | mouse | |
dc.subject | nerve cell membrane | |
dc.subject | neurite outgrowth | |
dc.subject | nonhuman | |
dc.subject | perikaryon | |
dc.subject | second messenger | |
dc.type | Article | |
dc.contributor.department | LIFE SCIENCES INSTITUTE | |
dc.description.doi | 10.3389/fncel.2016.00298 | |
dc.description.sourcetitle | Frontiers in Cellular Neuroscience | |
dc.description.volume | 10 | |
dc.description.page | 298 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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