MOLECULAR CLONING AND EXPRESSION OF AN OVINE CDNA ENCODING BOTH STEROID 11?-HYDROXYLASE AND ALDOSTERONE SYNTHASE ACTIVITES

Abstract

To fully understand the control of aldosterone secretion, it is necessary to identify the enzymes responsible for the enzymatic steps of converting the common steroid precursor cholesterol to aldosterone; especially the enzyme(s) (P45011? related enzymes) involved in the final steps which are 11?-hydroxylation, 18-hydroxylation and 18-oxidation. This thesis describes the characterisation of a cDNA clone corresponding to the ovine P45011?, both structurally and functionally, as well as the interpretations of results arising from this study and other laboratories