Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0041896
DC Field | Value | |
---|---|---|
dc.title | Oxidative stress-induced glomerular mineralocorticoid receptor activation limits the benefit of salt reduction in Dahl salt-sensitive rats | |
dc.contributor.author | Kitada K. | |
dc.contributor.author | Nakano D. | |
dc.contributor.author | Liu Y. | |
dc.contributor.author | Fujisawa Y. | |
dc.contributor.author | Hitomi H. | |
dc.contributor.author | Shibayama Y. | |
dc.contributor.author | Shibata H. | |
dc.contributor.author | Nagai Y. | |
dc.contributor.author | Mori H. | |
dc.contributor.author | Masaki T. | |
dc.contributor.author | Kobori H. | |
dc.contributor.author | Nishiyama A. | |
dc.date.accessioned | 2020-03-18T05:44:55Z | |
dc.date.available | 2020-03-18T05:44:55Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Kitada K., Nakano D., Liu Y., Fujisawa Y., Hitomi H., Shibayama Y., Shibata H., Nagai Y., Mori H., Masaki T., Kobori H., Nishiyama A. (2012). Oxidative stress-induced glomerular mineralocorticoid receptor activation limits the benefit of salt reduction in Dahl salt-sensitive rats. PLoS ONE 7 (7) : e41896. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0041896 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/165569 | |
dc.description.abstract | Background: Mineralocorticoid receptor (MR) antagonists attenuate renal injury in salt-sensitive hypertensive rats with low plasma aldosterone levels. We hypothesized that oxidative stress causes MR activation in high-salt-fed Dahl salt-sensitive rats. Furthermore, we determined if MR activation persisted and induced renal injury, even after switching from a high- to a normal-salt diet. Methods and Findings: High-salt feeding for 4 weeks increased dihydroethidium fluorescence (DHE, an oxidant production marker), p22phox (a NADPH oxidase subunit) and serum and glucocorticoid-regulated kinase-1 (SGK1, an MR transcript) in glomeruli, compared with normal-salt feeding, and these changes persisted 4 weeks after salt withdrawal. Tempol treatment (0.5 mmol/L) during high-salt feeding abolished the changes in DHE fluorescence, p22phox and SGK1. Dietary salt reduction after a 4-week high-salt diet decreased both blood pressure and proteinuria, but was associated with significantly higher proteinuria than in normal control rats at 4 weeks after salt reduction. Administration of tempol during high-salt feeding, or eplerenone, an MR antagonist (100 mg/kg/day), started after salt reduction, recovered proteinuria to normal levels at 4 weeks after salt reduction. Paraquat, a reactive oxygen species generator, enhanced MR transcriptional activity in cultured rat mesangial cells and mouse podocytes. Conclusions: These results suggest that oxidative stress plays an important role in glomerular MR activation in Dahl salt-sensitive rats. Persistent MR activation even after reducing salt intake could limit the beneficial effects of salt restriction. © 2012 Kitada et al. | |
dc.publisher | Public Library of Science | |
dc.source | Unpaywall 20200320 | |
dc.subject | eplerenone | |
dc.subject | hydroethidine | |
dc.subject | mineralocorticoid receptor | |
dc.subject | paraquat | |
dc.subject | protein p22 | |
dc.subject | protein p22phox | |
dc.subject | serum and glucocorticoid regulated kinase 1 | |
dc.subject | tempol | |
dc.subject | unclassified drug | |
dc.subject | animal cell | |
dc.subject | animal experiment | |
dc.subject | animal tissue | |
dc.subject | article | |
dc.subject | blood pressure regulation | |
dc.subject | controlled study | |
dc.subject | Dahl salt sensitive rat | |
dc.subject | genetic transcription | |
dc.subject | glomerulus | |
dc.subject | high sodium intake | |
dc.subject | kidney injury | |
dc.subject | male | |
dc.subject | mesangium cell | |
dc.subject | nonhuman | |
dc.subject | oxidative stress | |
dc.subject | podocyte | |
dc.subject | protein function | |
dc.subject | proteinuria | |
dc.subject | receptor activation | |
dc.subject | sodium restriction | |
dc.subject | tissue level | |
dc.subject | Adrenalectomy | |
dc.subject | Aldosterone | |
dc.subject | Animals | |
dc.subject | Biological Markers | |
dc.subject | Blood Pressure | |
dc.subject | Cyclic N-Oxides | |
dc.subject | Ethidium | |
dc.subject | Feeding Behavior | |
dc.subject | Genes, Reporter | |
dc.subject | Immediate-Early Proteins | |
dc.subject | Kidney Glomerulus | |
dc.subject | Luciferases | |
dc.subject | Male | |
dc.subject | Mesangial Cells | |
dc.subject | Mice | |
dc.subject | Oxidative Stress | |
dc.subject | Podocytes | |
dc.subject | Protein-Serine-Threonine Kinases | |
dc.subject | Proteinuria | |
dc.subject | Rats | |
dc.subject | Rats, Inbred Dahl | |
dc.subject | Receptors, Mineralocorticoid | |
dc.subject | Sodium Chloride, Dietary | |
dc.subject | Sodium-Hydrogen Antiporter | |
dc.subject | Spin Labels | |
dc.subject | Systole | |
dc.subject | Time Factors | |
dc.subject | Rattus | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1371/journal.pone.0041896 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 7 | |
dc.description.issue | 7 | |
dc.description.page | e41896 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1371_journal_pone_0041896.pdf | 494.25 kB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.