Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbi.2018.09.001
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dc.titleEvidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke
dc.contributor.authorPoh, Luting
dc.contributor.authorKang, Sung-Wook
dc.contributor.authorBaik, Sang-Ha
dc.contributor.authorNg, Gavin Yong Quan
dc.contributor.authorShe, David T
dc.contributor.authorBalaganapathy, Priyanka
dc.contributor.authorDheen, S Thameem
dc.contributor.authorMagnus, Tim
dc.contributor.authorGelderblom, Mathias
dc.contributor.authorSobey, Christopher G
dc.contributor.authorKoo, Edward H
dc.contributor.authorFann, David Y
dc.contributor.authorArumugam, Thiruma V
dc.date.accessioned2020-01-20T06:24:32Z
dc.date.available2020-01-20T06:24:32Z
dc.date.issued2019-01-01
dc.identifier.citationPoh, Luting, Kang, Sung-Wook, Baik, Sang-Ha, Ng, Gavin Yong Quan, She, David T, Balaganapathy, Priyanka, Dheen, S Thameem, Magnus, Tim, Gelderblom, Mathias, Sobey, Christopher G, Koo, Edward H, Fann, David Y, Arumugam, Thiruma V (2019-01-01). Evidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke. BRAIN BEHAVIOR AND IMMUNITY 75 : 34-47. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbi.2018.09.001
dc.identifier.issn0889-1591
dc.identifier.issn1090-2139
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/163877
dc.description.abstractStroke is the second leading cause of death in the world and a major cause of long-term disability. Recent evidence has provided insight into a newly described inflammatory mechanism that contributes to neuronal and glial cell death, and impaired neurological outcome following ischemic stroke – a form of sterile inflammation involving innate immune complexes termed inflammasomes. It has been established that inflammasome activation following ischemic stroke contributes to neuronal cell death, but little is known about inflammasome function and cell death in activated microglial cells following cerebral ischemia. Microglia are considered the resident immune cells that function as the primary immune defense in the brain. This study has comprehensively investigated the expression and activation of NLRP1, NLRP3, NLRC4 and AIM2 inflammasomes in isolates of microglial cells subjected to simulated ischemic conditions and in the brain following ischemic stroke. Immunoblot analysis from culture media indicated microglial cells release inflammasome components and inflammasome activation-dependent pro-inflammatory cytokines following ischemic conditions. In addition, a functional role for NLRC4 inflammasomes was determined using siRNA knockdown of NLRC4 and pharmacological inhibitors of caspase-1 and -8 to target apoptotic and pyroptotic cell death in BV2 microglial cells under ischemic conditions. In summary, the present study provides evidence that the NLRC4 inflammasome complex mediates the inflammatory response, as well as apoptotic and pyroptotic cell death in microglial cells under in vitro and in vivo ischemic conditions. © 2018 Elsevier Inc.
dc.language.isoen
dc.publisherElsevier
dc.sourceElements
dc.subjectApoptosis
dc.subjectCell death
dc.subjectInflammasomes
dc.subjectInflammation
dc.subjectIschemic stroke
dc.subjectMicroglia
dc.subjectNLRC4
dc.subjectPyroptosis
dc.typeArticle
dc.date.updated2020-01-17T06:37:21Z
dc.contributor.departmentANATOMY
dc.contributor.departmentMEDICINE
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/j.bbi.2018.09.001
dc.description.sourcetitleBRAIN BEHAVIOR AND IMMUNITY
dc.description.volume75
dc.description.page34-47
dc.description.codenBBIME
dc.published.statePublished
dc.grant.idNMRC-CBRG-0102/2016
dc.grant.idNMRC/OFIRG/0036/2017
dc.grant.fundingagencySingapore National Medical Research Council Research Grants
dc.grant.fundingagencyNational University of Singapore
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