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https://doi.org/10.1371/journal.ppat.1007157
DC Field | Value | |
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dc.title | Neutrophils use superoxide to control bacterial infection at a distance | |
dc.contributor.author | Phan Q.T. | |
dc.contributor.author | Sipka T. | |
dc.contributor.author | Gonzalez C. | |
dc.contributor.author | Levraud J.-P. | |
dc.contributor.author | Lutfalla G. | |
dc.contributor.author | Nguyen-Chi M. | |
dc.date.accessioned | 2019-11-08T06:42:08Z | |
dc.date.available | 2019-11-08T06:42:08Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Phan Q.T., Sipka T., Gonzalez C., Levraud J.-P., Lutfalla G., Nguyen-Chi M. (2018). Neutrophils use superoxide to control bacterial infection at a distance. PLoS Pathogens 14 (7) : e1007157. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1007157 | |
dc.identifier.issn | 15537366 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161882 | |
dc.description.abstract | Understanding the roles of neutrophils and macrophages in fighting bacterial infections is a critical issue in human pathologies. Although phagocytic killing has been extensively studied, little is known about how bacteria are eliminated extracellularly in live vertebrates. We have recently developed an infection model in the zebrafish embryo in which leukocytes cannot reach the injected bacteria. When Escherichia coli bacteria are injected within the notochord, both neutrophils and macrophages are massively recruited during several days, but do not infiltrate the infected tissue presumably because of its tough collagen sheath. Nevertheless, the bacteria are killed during the first 24 hours, and we report here that neutrophils, but not macrophages are involved in the control of the infection. Using genetic and chemical approaches, we show that even in absence of phagocytosis, the bactericidal action relies on NADPH oxidase-dependent production of superoxide in neutrophils. We thus reveal a host effector mechanism mediated by neutrophils that eliminates bacteria that cannot be reached by phagocytes and that is independent of macrophages, NO synthase or myeloperoxidase. ? 2018 Phan et al. http://creativecommons.org/licenses/by/4.0/. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | collagen | |
dc.subject | messenger RNA | |
dc.subject | metronidazole | |
dc.subject | reduced nicotinamide adenine dinucleotide phosphate oxidase | |
dc.subject | superoxide | |
dc.subject | vas 2870 | |
dc.subject | superoxide | |
dc.subject | Article | |
dc.subject | bacterial clearance | |
dc.subject | bacterial infection | |
dc.subject | cell population | |
dc.subject | colony forming unit | |
dc.subject | confocal microscopy | |
dc.subject | controlled study | |
dc.subject | embryo | |
dc.subject | enzyme activity | |
dc.subject | Escherichia coli | |
dc.subject | fluorescence microscopy | |
dc.subject | immunohistochemistry | |
dc.subject | infection control | |
dc.subject | larva | |
dc.subject | leukocyte | |
dc.subject | macrophage | |
dc.subject | neutrophil | |
dc.subject | nonhuman | |
dc.subject | notochord | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | Salmonella enterica serovar Typhimurium | |
dc.subject | zebra fish | |
dc.subject | animal | |
dc.subject | Escherichia coli infection | |
dc.subject | immunology | |
dc.subject | neutrophil | |
dc.subject | Animals | |
dc.subject | Escherichia coli | |
dc.subject | Escherichia coli Infections | |
dc.subject | Neutrophils | |
dc.subject | Superoxides | |
dc.subject | Zebrafish | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1371/journal.ppat.1007157 | |
dc.description.sourcetitle | PLoS Pathogens | |
dc.description.volume | 14 | |
dc.description.issue | 7 | |
dc.description.page | e1007157 | |
Appears in Collections: | Elements Staff Publications |
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