Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0004104
DC FieldValue
dc.titleNematode homologue of PQBP1, a mental retardation causative gene, is involved in lipid metabolism
dc.contributor.authorTakahashi K.
dc.contributor.authorYoshina S.
dc.contributor.authorMasashi M.
dc.contributor.authorIto W.
dc.contributor.authorInoue T.
dc.contributor.authorShiwaku H.
dc.contributor.authorArai H.
dc.contributor.authorMitani S.
dc.contributor.authorOkazawa H.
dc.date.accessioned2019-11-08T00:54:35Z
dc.date.available2019-11-08T00:54:35Z
dc.date.issued2009
dc.identifier.citationTakahashi K., Yoshina S., Masashi M., Ito W., Inoue T., Shiwaku H., Arai H., Mitani S., Okazawa H. (2009). Nematode homologue of PQBP1, a mental retardation causative gene, is involved in lipid metabolism. PLoS ONE 4 (1) : e4104. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0004104
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161845
dc.description.abstractBackground: PQBP1 is a causative gene for X-linked mental retardation (MR) whose patients frequently show lean body. C. elegans has a strictly conserved homologue gene of PQBP1, T21D12.3. Methodology and Principal Findings: We generated Venus-transgenic and T21D12.3-mutant nematodes to analyze developmental expression patterns and in vivo functions of the nematode PQBP1 homologue protein (pqbp-1.1). During development, pqbp-1.1 is expressed from cell proliferation stage to larva stage. In larva, intestinal cells show the highest expression of pqbp-1.1, while it decreases in adult worms. The mutants of pqbp-1.1 show a decrease of the lipid content in intestinal cells. Especially, incorporation of fatty acid into triglyceride is impaired. ShRNA-mediated repression of PQBP1 also leads to reduction of lipid content in mammalian primary white adipocytes. Conclusion/Significance: These results suggest that pqbp-1.1 is involved in lipid metabolism of intestinal cells. Dysfunction of lipid metabolism might underlie lean body, one of the most frequent symptoms associating with PQBP1-linked MR patients. � 2009 Takahashi et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectbinding protein
dc.subjectfatty acid
dc.subjectpolyglutamate binding protein
dc.subjectprotein PQBP1
dc.subjectshort hairpin RNA
dc.subjecttriacylglycerol
dc.subjectunclassified drug
dc.subjectCaenorhabditis elegans protein
dc.subjectcarrier protein
dc.subjecthybrid protein
dc.subjectnuclear protein
dc.subjectPQBP1 protein, human
dc.subjectadipocyte
dc.subjectarticle
dc.subjectCaenorhabditis elegans
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectevolutionary homology
dc.subjectgene expression
dc.subjectgene function
dc.subjectgene repression
dc.subjectin vivo study
dc.subjectintestine cell
dc.subjectlarval stage
dc.subjectlipid analysis
dc.subjectlipid metabolism
dc.subjectmutant
dc.subjectnonhuman
dc.subjectprotein defect
dc.subjectprotein function
dc.subjecttransgenics
dc.subjectanimal
dc.subjectCaenorhabditis elegans
dc.subjectcell culture
dc.subjectcytology
dc.subjectgenetics
dc.subjecthistology
dc.subjecthuman
dc.subjectintestine
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectsyndrome
dc.subjecttransgenic animal
dc.subjectwhite adipose tissue
dc.subjectX linked mental retardation
dc.subjectCaenorhabditis elegans
dc.subjectMammalia
dc.subjectNematoda
dc.subjectAdipocytes
dc.subjectAdipose Tissue, White
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectCaenorhabditis elegans
dc.subjectCaenorhabditis elegans Proteins
dc.subjectCarrier Proteins
dc.subjectCells, Cultured
dc.subjectHumans
dc.subjectIntestines
dc.subjectLipid Metabolism
dc.subjectMental Retardation, X-Linked
dc.subjectNuclear Proteins
dc.subjectRecombinant Fusion Proteins
dc.subjectSyndrome
dc.typeArticle
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.1371/journal.pone.0004104
dc.description.sourcetitlePLoS ONE
dc.description.volume4
dc.description.issue1
dc.description.pagee4104
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0004104.pdf450.42 kBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

16
checked on Mar 6, 2021

Page view(s)

110
checked on Mar 5, 2021

Download(s)

1
checked on Mar 5, 2021

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons