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https://doi.org/10.1371/journal.pone.0015341
DC Field | Value | |
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dc.title | CSPG is a secreted factor that stimulates neural stem cell survival possibly by enhanced EGFR signaling | |
dc.contributor.author | Tham M. | |
dc.contributor.author | Ramasamy S. | |
dc.contributor.author | Gan H.T. | |
dc.contributor.author | Ramachandran A. | |
dc.contributor.author | Poonepalli A. | |
dc.contributor.author | Yu Y.H. | |
dc.contributor.author | Ahmed S. | |
dc.date.accessioned | 2019-11-07T07:59:20Z | |
dc.date.available | 2019-11-07T07:59:20Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Tham M., Ramasamy S., Gan H.T., Ramachandran A., Poonepalli A., Yu Y.H., Ahmed S. (2010). CSPG is a secreted factor that stimulates neural stem cell survival possibly by enhanced EGFR signaling. PLoS ONE 5 (12) : e15341. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0015341 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161799 | |
dc.description.abstract | Understanding how autocrine/paracrine factors regulate neural stem cell (NSC) survival and growth is fundamental to the utilization of these cells for therapeutic applications and as cellular models for the brain. In vitro, NSCs can be propagated along with neural progenitors (NPs) as neurospheres (nsphs). The nsph conditioned medium (nsph-CM) contains cellsecreted factors that can regulate NSC behavior. However, the identity and exact function of these factors within the nsph- CM has remained elusive. We analyzed the nsph-CM by mass spectrometry and identified DSD-1-proteoglycan, a chondroitin sulfate proteoglycan (CSPG), apolipoprotein E (ApoE) and cystatin C as components of the nsph-CM. Using clonal assays we show that CSPG and ApoE are responsible for the ability of the nsph-CM to stimulate nsph formation whereas cystatin C is not involved. Clonal nsphs generated in the presence of CSPG show more than four-fold increase in NSCs. Thus CSPG specifically enhances the survival of NSCs. CSPG also stimulates the survival of embryonic stem cell (ESC)- derived NSCs, and thus may be involved in the developmental transition of ESCs to NSCs. In addition to its role in NSC survival, CSPG maintains the three dimensional structure of nsphs. Lastly, CSPG's effects on NSC survival may be mediated by enhanced signaling via EGFR, JAK/STAT3 and PI3K/Akt pathways. © 2010 Tham et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | apolipoprotein E | |
dc.subject | cystatin C | |
dc.subject | epidermal growth factor receptor | |
dc.subject | Janus kinase | |
dc.subject | phosphatidylinositol 3 kinase | |
dc.subject | protein kinase B | |
dc.subject | proteochondroitin sulfate | |
dc.subject | proteoglycan | |
dc.subject | STAT3 protein | |
dc.subject | apolipoprotein E | |
dc.subject | cystatin C | |
dc.subject | epidermal growth factor receptor | |
dc.subject | phosphatidylinositol 3 kinase | |
dc.subject | proteochondroitin sulfate | |
dc.subject | animal cell | |
dc.subject | article | |
dc.subject | cell differentiation | |
dc.subject | cell proliferation | |
dc.subject | cell survival | |
dc.subject | controlled study | |
dc.subject | embryonic stem cell | |
dc.subject | immunocytochemistry | |
dc.subject | in vitro study | |
dc.subject | mass spectrometry | |
dc.subject | mouse | |
dc.subject | neural stem cell | |
dc.subject | nonhuman | |
dc.subject | protein analysis | |
dc.subject | protein structure | |
dc.subject | signal transduction | |
dc.subject | structure analysis | |
dc.subject | animal | |
dc.subject | C57BL mouse | |
dc.subject | cell survival | |
dc.subject | culture medium | |
dc.subject | cytology | |
dc.subject | immunohistochemistry | |
dc.subject | metabolism | |
dc.subject | methodology | |
dc.subject | nerve cell | |
dc.subject | neural stem cell | |
dc.subject | signal transduction | |
dc.subject | stem cell | |
dc.subject | Animals | |
dc.subject | Apolipoproteins E | |
dc.subject | Cell Proliferation | |
dc.subject | Cell Survival | |
dc.subject | Chondroitin Sulfate Proteoglycans | |
dc.subject | Culture Media, Conditioned | |
dc.subject | Cystatin C | |
dc.subject | Embryonic Stem Cells | |
dc.subject | Immunohistochemistry | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Neural Stem Cells | |
dc.subject | Neurons | |
dc.subject | Phosphatidylinositol 3-Kinases | |
dc.subject | Receptor, Epidermal Growth Factor | |
dc.subject | Signal Transduction | |
dc.subject | Stem Cells | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.1371/journal.pone.0015341 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 5 | |
dc.description.issue | 12 | |
dc.description.page | e15341 | |
Appears in Collections: | Elements Staff Publications |
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