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https://doi.org/10.1371/journal.pone.0110668
Title: | BMP Signaling in astrocytes downregulates EGFR to modulate survival and maturation | Authors: | Scholze A.R. Foo L.C. Mulinyawe S. Barres B.A. |
Keywords: | aquaporin 4 bone morphogenetic protein bone morphogenetic protein 5 epidermal growth factor receptor fibroblast growth factor glial fibrillary acidic protein protein S100B Smad1 protein Smad5 protein Smad8 protein somatomedin B Wnt7a protein bone morphogenetic protein Egfr protein, rat epidermal growth factor receptor animal cell animal tissue Article astrocyte cell differentiation cell heterogeneity cell lineage cell maturation cell proliferation cell survival controlled study down regulation immunoblotting immunoreactivity in vitro study nonhuman oligodendroglia prospective study protein analysis protein expression protein phosphorylation purification rat signal transduction upregulation animal astrocyte biosynthesis gene expression regulation genetics metabolism mouse phosphorylation physiology signal transduction Animals Astrocytes Bone Morphogenetic Proteins Gene Expression Regulation, Developmental Mice Phosphorylation Rats Receptor, Epidermal Growth Factor Signal Transduction |
Issue Date: | 2014 | Citation: | Scholze A.R., Foo L.C., Mulinyawe S., Barres B.A. (2014). BMP Signaling in astrocytes downregulates EGFR to modulate survival and maturation. PLoS ONE 9 (10) : e110668. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0110668 | Rights: | Attribution 4.0 International | Abstract: | Astrocytes constitute a major cell population in the brain with a myriad of essential functions, yet we know remarkably little about the signaling pathways and mechanisms that direct astrocyte maturation. To explore the signals regulating astrocyte development, we prospectively purified and cultured immature postnatal rodent astrocytes. We identified fibroblast growth factors (FGFs) and bone morphogenetic proteins (BMPs) as robust trophic factors for immature astrocytes. We showed that astrocytes respond directly to BMPs via phosphorylation of the smad1/5/8 pathway. In vitro, BMP signaling promoted immature astrocytes to adopt multiple characteristics of mature astrocytes, including a more process-bearing morphology, aquaporin-4 (AQP4) and S100? immunoreactivity, limited proliferation, and strong downregulation of epidermal growth factor receptor (EGFR). In vivo, activation of the smad1/5/8 pathway in astrocytes was seen during early postnatal development, but inhibition of astrocyte proliferation was not observed. These insights can aid in the further dissection of the mechanisms and pathways controlling astrocyte biology and development. © 2014 Scholze et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161767 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0110668 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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