Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0136254
DC Field | Value | |
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dc.title | Epidemiological and evolutionary dynamics of influenza B viruses in Malaysia, 2012-2014 | |
dc.contributor.author | Oong X.Y. | |
dc.contributor.author | Ng K.T. | |
dc.contributor.author | Lam T.T.-Y. | |
dc.contributor.author | Pang Y.K. | |
dc.contributor.author | Chan K.G. | |
dc.contributor.author | Hanafi N.S. | |
dc.contributor.author | Kamarulzaman A. | |
dc.contributor.author | Tee K.K. | |
dc.date.accessioned | 2019-11-06T01:27:15Z | |
dc.date.available | 2019-11-06T01:27:15Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Oong X.Y., Ng K.T., Lam T.T.-Y., Pang Y.K., Chan K.G., Hanafi N.S., Kamarulzaman A., Tee K.K. (2015). Epidemiological and evolutionary dynamics of influenza B viruses in Malaysia, 2012-2014. PLoS ONE 10 (8) : e0136254. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0136254 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161490 | |
dc.description.abstract | Epidemiological and evolutionary dynamics of influenza B Victoria and Yamagata lineages remained poorly understood in the tropical Southeast Asia region, despite causing seasonal outbreaks worldwide. From 2012-2014, nasopharyngeal swab samples collected from outpatients experiencing acute upper respiratory tract infection symptoms in Kuala Lumpur, Malaysia, were screened for influenza viruses using a multiplex RT-PCR assay. Among 2,010/3,935 (51.1%) patients infected with at least one respiratory virus, 287 (14.3%) and 183 (9.1%) samples were tested positive for influenza A and B viruses, respectively. Influenza-positive cases correlate significantly with meteorological factors - total amount of rainfall, relative humidity, number of rain days, ground temperature and particulate matter (PM10). Phylogenetic reconstruction of haemagglutinin (HA) gene from 168 influenza B viruses grouped them into Yamagata Clade 3 (65, 38.7%), Yamagata Clade 2 (48, 28.6%) and Victoria Clade 1 (55, 32.7%). With neuraminidase (NA) phylogeny, 30 intra-clade (29 within Yamagata Clade 3, 1 within Victoria Clade 1) and 1 inter-clade (Yamagata Clade 2-HA/Yamagata Clade 3-NA) reassortants were identified. Study of virus temporal dynamics revealed a lineage shift from Victoria to Yamagata (2012-2013), and a clade shift from Yamagata Clade 2 to Clade 3 (2013-2014). Yamagata Clade 3 predominating in 2014 consisted of intra-clade reassortants that were closely related to a recent WHO vaccine candidate strain (B/Phuket/3073/2013), with the reassortment event occurred approximately 2 years ago based on Bayesian molecular clock estimation. Malaysian Victoria Clade 1 viruses carried H274Y substitution in the active site of neuraminidase, which confers resistance to oseltamivir. Statistical analyses on clinical and demographic data showed Yamagata-infected patients were older and more likely to experience headache while Victoriainfected patients were more likely to experience nasal congestion and sore throat. This study describes the evolution of influenza B viruses in Malaysia and highlights the importance of continuous surveillance for better vaccination policy in this region. © 2015 Oong et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | hemagglutinin | |
dc.subject | oseltamivir | |
dc.subject | rain | |
dc.subject | sialidase | |
dc.subject | Influenza virus hemagglutinin | |
dc.subject | sialidase | |
dc.subject | adult | |
dc.subject | Article | |
dc.subject | cladistics | |
dc.subject | controlled study | |
dc.subject | environmental temperature | |
dc.subject | enzyme active site | |
dc.subject | epidemic | |
dc.subject | headache | |
dc.subject | human | |
dc.subject | humidity | |
dc.subject | influenza A | |
dc.subject | influenza B | |
dc.subject | Malaysia | |
dc.subject | nonhuman | |
dc.subject | nose obstruction | |
dc.subject | outpatient | |
dc.subject | particulate matter | |
dc.subject | phylogeny | |
dc.subject | respiratory tract infection | |
dc.subject | sore throat | |
dc.subject | vaccination | |
dc.subject | virus strain | |
dc.subject | adolescent | |
dc.subject | amino acid substitution | |
dc.subject | clinical trial | |
dc.subject | female | |
dc.subject | genetics | |
dc.subject | Influenza B virus | |
dc.subject | Influenza, Human | |
dc.subject | male | |
dc.subject | middle aged | |
dc.subject | missense mutation | |
dc.subject | molecular evolution | |
dc.subject | multiplex polymerase chain reaction | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | Adolescent | |
dc.subject | Adult | |
dc.subject | Amino Acid Substitution | |
dc.subject | Evolution, Molecular | |
dc.subject | Female | |
dc.subject | Hemagglutinin Glycoproteins, Influenza Virus | |
dc.subject | Humans | |
dc.subject | Influenza B virus | |
dc.subject | Influenza, Human | |
dc.subject | Malaysia | |
dc.subject | Male | |
dc.subject | Middle Aged | |
dc.subject | Multiplex Polymerase Chain Reaction | |
dc.subject | Mutation, Missense | |
dc.subject | Neuraminidase | |
dc.subject | Phylogeny | |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1371/journal.pone.0136254 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 10 | |
dc.description.issue | 8 | |
dc.description.page | e0136254 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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