Please use this identifier to cite or link to this item: https://doi.org/10.1158/0008-5472.CAN-13-2309
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dc.titleCD44v8-10 Is a Cancer-Specific Marker for Gastric Cancer Stem Cells
dc.contributor.authorLau, Wen Min
dc.contributor.authorTeng, Eileen
dc.contributor.authorChong, Hui Shan
dc.contributor.authorLopez, Kirsten Anne Pagaduan
dc.contributor.authorTay, Amy Yuh Ling
dc.contributor.authorSalto-Tellez, Manuel
dc.contributor.authorShabbir, Asim
dc.contributor.authorSo, Jimmy Bok Yan
dc.contributor.authorChan, Shing Leng
dc.date.accessioned2019-06-03T04:08:47Z
dc.date.available2019-06-03T04:08:47Z
dc.date.issued2014-05-01
dc.identifier.citationLau, Wen Min, Teng, Eileen, Chong, Hui Shan, Lopez, Kirsten Anne Pagaduan, Tay, Amy Yuh Ling, Salto-Tellez, Manuel, Shabbir, Asim, So, Jimmy Bok Yan, Chan, Shing Leng (2014-05-01). CD44v8-10 Is a Cancer-Specific Marker for Gastric Cancer Stem Cells. CANCER RESEARCH 74 (9) : 2630-2641. ScholarBank@NUS Repository. https://doi.org/10.1158/0008-5472.CAN-13-2309
dc.identifier.issn0008-5472
dc.identifier.issn1538-7445
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/155006
dc.description.abstractThe surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression in many cell types, including hematopoietic cells, has hindered its use in targeting CSCs. In this study, 28 paired primary tumor and adjacent nontumor gastric tissue samples were analyzed for cell surface protein expression. Cells that expressed pan-CD44 were found to occur at significantly higher frequency in gastric tumor tissues. We identified CD44v8-10 as the predominant CD44 variant expressed in gastric cancer cells and verified its role as a gastric CSC marker by limiting dilution and serial transplantation assays. Parallel experiments using CD133 failed to enrich for gastric CSCs. Analyses of another 26 primary samples showed significant CD44v8-10 upregulation in gastric tumor sites. Exogenous expression of CD44v8-10 but not CD44 standard (CD44s) increased the frequency of tumor initiation in immunocompromised mice. Reciprocal silencing of total CD44 resulted in reduced tumor-initiating potential of gastric cancer cells that could be rescued by CD44v8-10 but not CD44s expression. Our findings provide important functional evidence that CD44v8-10 marks human gastric CSCs and contributes to tumor initiation, possibly through enhancing oxidative stress defense. In addition, we showed that CD44v8-10 expression is low in normal tissues. Because CD44 also marks CSCs of numerous human cancers, many of which may also overexpress CD44v8-10, CD44v8-10 may provide an avenue to target CSCs in other human cancers. © 2014 AACR.
dc.language.isoen
dc.publisherAMER ASSOC CANCER RESEARCH
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectOncology
dc.subjectCD44 VARIANT PROTEINS
dc.subjectDE-NOVO EXPRESSION
dc.subjectADHESION MOLECULE
dc.subjectSIDE POPULATION
dc.subjectTUMOR-GROWTH
dc.subjectADENOCARCINOMA
dc.subjectCARCINOMA
dc.subjectIDENTIFICATION
dc.subjectTUMORIGENESIS
dc.subjectGLYCOPROTEIN
dc.typeArticle
dc.date.updated2019-06-03T02:42:57Z
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentDEPT OF SURGERY
dc.description.doi10.1158/0008-5472.CAN-13-2309
dc.description.sourcetitleCANCER RESEARCH
dc.description.volume74
dc.description.issue9
dc.description.page2630-2641
dc.description.placeUnited States
dc.published.statePublished
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