Please use this identifier to cite or link to this item:
|Title:||NGAL is downregulated in oral squamous cell carcinoma and leads to increased survival, proliferation, migration and chemoresistance||Authors:||Monisha J.
|Keywords:||Drug resistance; mTOR pathway; Oral cancer; Secreted glycoprotein||Issue Date:||2018||Publisher:||MDPI AG||Citation:||Monisha J., Roy N.K., Padmavathi G., Banik K., Bordoloi D., Khwairakpam A.D., Arfuso F., Chinnathambi A., Alahmadi T.A., Alharbi S.A., Sethi G., Kumar A.P., Kunnumakkara A.B. (2018). NGAL is downregulated in oral squamous cell carcinoma and leads to increased survival, proliferation, migration and chemoresistance. Cancers 10 (7) : 228. ScholarBank@NUS Repository. https://doi.org/10.3390/cancers10070228||Abstract:||Oral cancer is a major public health burden worldwide. The lack of biomarkers for early diagnosis has increased the difficulty in managing this disease. Recent studies have reported that neutrophil gelatinase-associated lipocalin (NGAL), a secreted glycoprotein, is upregulated in various tumors. In our study, we found that NGAL was significantly downregulated in primary malignant and metastatic tissues of oral cancer in comparison to normal tissues. The downregulation of NGAL was strongly correlated with both degree of differentiation and stage (I–IV); it can also serve as a prognostic biomarker for oral cancer. Additionally, tobacco carcinogens were found to be involved in the downregulation of NGAL. Mechanistic studies revealed that knockdown of NGAL increased oral cancer cell proliferation, survival, and migration; it also induced resistance against cisplatin. Silencing of NGAL activated mammalian target of rapamycin (mTOR)signaling and reduced autophagy by the liver kinase B1 (LKB1)-activated protein kinase (AMPK)-p53-Redd1 signaling axis. Moreover, cyclin-D1, Bcl-2, and matrix metalloproteinase-9 (MMP-9) were upregulated, and caspase-9 was downregulated, suggesting that silencing of NGAL increases oral cancer cell proliferation, survival, and migration. Thus, from our study, it is evident that downregulation of NGAL activates the mTOR pathway and helps in the progression of oral cancer. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.||Source Title:||Cancers||URI:||http://scholarbank.nus.edu.sg/handle/10635/152462||ISSN:||20726694||DOI:||10.3390/cancers10070228|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
|cancers10070228.pdf||3.05 MB||Adobe PDF|
checked on Sep 21, 2020
WEB OF SCIENCETM
checked on Jul 16, 2019
checked on Sep 18, 2020
checked on Sep 18, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.