Please use this identifier to cite or link to this item:
https://doi.org/10.1167/iovs.17-22694
DC Field | Value | |
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dc.title | Bevacizumab Promotes T-Cell-Mediated Collagen Deposition in the Mouse Model of Conjunctival Scarring | |
dc.contributor.author | Seet L.-F. | |
dc.contributor.author | Toh L.Z. | |
dc.contributor.author | Chu S. | |
dc.contributor.author | Finger S.N. | |
dc.contributor.author | Ginhoux F. | |
dc.contributor.author | Hong W. | |
dc.contributor.author | Wong T.T. | |
dc.date.accessioned | 2019-03-12T08:10:22Z | |
dc.date.available | 2019-03-12T08:10:22Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Seet L.-F., Toh L.Z., Chu S., Finger S.N., Ginhoux F., Hong W., Wong T.T. (2018). Bevacizumab Promotes T-Cell-Mediated Collagen Deposition in the Mouse Model of Conjunctival Scarring. Investigative ophthalmology & visual science 59 (3) : 1682-1692. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.17-22694 | |
dc.identifier.issn | 15525783 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/152204 | |
dc.description.abstract | Purpose: We determine the effects of bevacizumab on collagen production in a mouse model of conjunctival scarring.Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring, and bevacizumab was introduced by conjunctival injection. The capacity of bevacizumab to recognize conjunctival VEGF-A was determined by ELISA. Col1a1 was measured by real-time PCR and immunoblotting. T cells and collagen were visualized by immunofluorescence and picrosirius red staining of bleb cryosections. Conjunctival CD4+ or CD8a+ T cells were counted by flow cytometry. Mouse splenic T cells were cultured with bevacizumab/IgG and their numbers, cell cycle, and collagen production were measured using a cell counter, flow cytometry, and sircol soluble collagen assay, respectively. Reconstitution experiments in severe combined immunodeficiency (SCID) mice were performed by injection of freshly isolated T cells on day 2 postoperatively.Results: Bevacizumab recognized approximately 20% of endogenous murine VEGF-A. Injection of bevacizumab raised Col1a1 expression in the blebs at mRNA and protein levels. Bevacizumab did not induce collagen in conjunctival fibroblasts, but increased CD4+ and CD8a+ cell numbers as well as collagen production by these cells. Collagen appeared to accumulate in the vicinity of T cells in the bevacizumab-treated blebs. While SCID blebs did not show elevated collagen levels, reconstitution with CD4+ or CD8a+ cells resulted in increased Col1a1 expression at mRNA and protein levels.Conclusions: Bevacizumab increased collagen production in the mouse model of conjunctival scarring. This collagen induction was mediated by T cells that were also stimulated by bevacizumab to increase in numbers. | |
dc.source | Scopus | |
dc.subject | bevacizumab | |
dc.subject | collagen | |
dc.subject | conjunctiva | |
dc.subject | T cells | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1167/iovs.17-22694 | |
dc.description.sourcetitle | Investigative ophthalmology & visual science | |
dc.description.volume | 59 | |
dc.description.issue | 3 | |
dc.description.page | 1682-1692 | |
dc.published.state | published | |
dc.grant.id | NMRC/CG/015/2013 | |
dc.grant.fundingagency | Singapore National Research Foundation | |
dc.grant.fundingagency | Singapore Ministry of Health抯 National Medical Research Council | |
Appears in Collections: | Elements Staff Publications |
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