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Title: | Microfluidics and microarray based approaches to biological analysis | Authors: | LESAICHERRE MARIE LAURE | Keywords: | Microfluidics microarray DNA protein kinase PCR | Issue Date: | 6-Jun-2005 | Citation: | LESAICHERRE MARIE LAURE (2005-06-06). Microfluidics and microarray based approaches to biological analysis. ScholarBank@NUS Repository. | Abstract: | In the first part of this work, microfabricated Capillary Electrophoresis devices were integrated with microfabricated PCR devices for fast DNA amplification and separation., rapid on-chip amplification and separation of genes of interest was performed. A micro DNA extraction device using paramagnetic micro silica beads was consequently developed, subsequently a combined on-chip DNA extraction from a single drop of blood and PCR amplification were performed. In the second part of this work, micro array-based strategies for proteomics studies were developed. Two new strategies for site-specific immobilization of kinase substrates onto functionalized slides were developed: In addition, a novel fluorescent antibody-based detection was developed to rapidly screen for kinase substrate phosphorylation. Avidin-biotin is one of the strongest know non-covalent interaction, and by arraying C-terminal biotinylated proteins on avidin functionalized slides, a very stable array of functionally active proteins was also obtained. | URI: | http://scholarbank.nus.edu.sg/handle/10635/14779 |
Appears in Collections: | Ph.D Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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Acknowledgments.pdf | 53.81 kB | Adobe PDF | OPEN | None | View/Download | |
Summary.pdf | 58.02 kB | Adobe PDF | OPEN | None | View/Download | |
Content.pdf | 122.14 kB | Adobe PDF | OPEN | None | View/Download | |
Chap 1 Intro.pdf | 248.93 kB | Adobe PDF | OPEN | None | View/Download | |
Chap 2 CE PCR.pdf | 1.23 MB | Adobe PDF | OPEN | None | View/Download | |
Chap 3 Kinase array.pdf | 453.36 kB | Adobe PDF | OPEN | None | View/Download | |
Chap 4 Intein.pdf | 375.33 kB | Adobe PDF | OPEN | None | View/Download | |
Chap 5 Conclusion.pdf | 66.62 kB | Adobe PDF | OPEN | None | View/Download | |
Publications.pdf | 87.54 kB | Adobe PDF | OPEN | None | View/Download | |
First page.pdf | 43.96 kB | Adobe PDF | OPEN | None | View/Download | |
Second page.pdf | 32.23 kB | Adobe PDF | OPEN | None | View/Download |
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