Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14779
Title: Microfluidics and microarray based approaches to biological analysis
Authors: LESAICHERRE MARIE LAURE
Keywords: Microfluidics microarray DNA protein kinase PCR
Issue Date: 6-Jun-2005
Source: LESAICHERRE MARIE LAURE (2005-06-06). Microfluidics and microarray based approaches to biological analysis. ScholarBank@NUS Repository.
Abstract: In the first part of this work, microfabricated Capillary Electrophoresis devices were integrated with microfabricated PCR devices for fast DNA amplification and separation., rapid on-chip amplification and separation of genes of interest was performed. A micro DNA extraction device using paramagnetic micro silica beads was consequently developed, subsequently a combined on-chip DNA extraction from a single drop of blood and PCR amplification were performed. In the second part of this work, micro array-based strategies for proteomics studies were developed. Two new strategies for site-specific immobilization of kinase substrates onto functionalized slides were developed: In addition, a novel fluorescent antibody-based detection was developed to rapidly screen for kinase substrate phosphorylation. Avidin-biotin is one of the strongest know non-covalent interaction, and by arraying C-terminal biotinylated proteins on avidin functionalized slides, a very stable array of functionally active proteins was also obtained.
URI: http://scholarbank.nus.edu.sg/handle/10635/14779
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Acknowledgments.pdf53.81 kBAdobe PDF

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Summary.pdf58.02 kBAdobe PDF

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Content.pdf122.14 kBAdobe PDF

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Chap 1 Intro.pdf248.93 kBAdobe PDF

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Chap 2 CE PCR.pdf1.23 MBAdobe PDF

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Chap 3 Kinase array.pdf453.36 kBAdobe PDF

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Chap 4 Intein.pdf375.33 kBAdobe PDF

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Chap 5 Conclusion.pdf66.62 kBAdobe PDF

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Publications.pdf87.54 kBAdobe PDF

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First page.pdf43.96 kBAdobe PDF

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Second page.pdf32.23 kBAdobe PDF

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