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dc.titleLowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles
dc.contributor.authorWANG JIONGWEI
dc.contributor.authorZHANG YANAN
dc.contributor.authorSze, Siu Kwan
dc.contributor.authorvan de Weg, Sander M
dc.contributor.authorVernooij, Flora
dc.contributor.authorSchoneveld, Arjan H
dc.contributor.authorTAN SOCK HWEE
dc.contributor.authorVersteeg, Henri H
dc.contributor.authorTimmers, Leo
dc.contributor.authorLAM SU PING,CAROLYN
dc.contributor.authorDOMINIQUE P.V. DE KLEIJN
dc.identifier.citationWANG JIONGWEI, ZHANG YANAN, Sze, Siu Kwan, van de Weg, Sander M, Vernooij, Flora, Schoneveld, Arjan H, TAN SOCK HWEE, Versteeg, Henri H, Timmers, Leo, LAM SU PING,CAROLYN, DOMINIQUE P.V. DE KLEIJN (2017-12-29). Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles. International Journal o f Molecular Sciences 19 (1). ScholarBank@NUS Repository.
dc.description.abstractPlasma extracellular vesicles (EVs) are lipid membrane vesicles involved in several biological processes including coagulation. Both coagulation and lipid metabolism are strongly associated with cardiovascular events. Lowering very-low- and low-density lipoprotein ((V)LDL) particles via dextran sulphate LDL apheresis also removes coagulation proteins. It remains unknown, however, how coagulation proteins are removed in apheresis. We hypothesize that plasma EVs that contain high levels of coagulation proteins are concomitantly removed with (V)LDL particles by dextran sulphate apheresis. For this, we precipitated (V)LDL particles from human plasma with dextran sulphate and analyzed the abundance of coagulation proteins and EVs in the precipitate. Coagulation pathway proteins, as demonstrated by proteomics and a bead-based immunoassay, were over-represented in the (V)LDL precipitate. In this precipitate, both bilayer EVs and monolayer (V)LDL particles were observed by electron microscopy. Separation of EVs from (V)LDL particles using density gradient centrifugation revealed that almost all coagulation proteins were present in the EVs and not in the (V)LDL particles. These EVs also showed a strong procoagulant activity. Our study suggests that dextran sulphate used in LDL apheresis may remove procoagulant EVs concomitantly with (V)LDL particles, leading to a loss of coagulation proteins from the blood.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.subjectLDL apheresis
dc.subjectextracellular vesicles
dc.subjectlipoprotein particles
dc.subjectlow-density lipoprotein
dc.contributor.departmentDEPT OF SURGERY
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.sourcetitleInternational Journal o f Molecular Sciences
dc.grant.idNUHS O-CRG 2016 Oct-23
dc.grant.fundingagencyNational University Health System
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