Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep37235
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dc.titleNitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta
dc.contributor.authorTan, Kenneth Wei Min
dc.contributor.authorLin, Huixin
dc.contributor.authorShen, Hui
dc.contributor.authorLee, Yuan Kun
dc.date.accessioned2016-12-13T03:17:48Z
dc.date.available2016-12-13T03:17:48Z
dc.date.issued2016-11-16
dc.identifier.citationTan, Kenneth Wei Min, Lin, Huixin, Shen, Hui, Lee, Yuan Kun (2016-11-16). Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta. Scientific Reports 6 : 1-13. ScholarBank@NUS Repository. https://doi.org/10.1038/srep37235
dc.identifier.issn20452322
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132280
dc.description.abstractCertain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this study, the microalga Dunaliella tertiolecta was used as a model to study the global gene expression profile regulating starch accumulation in microalgae. D. tertiolecta, when depleted of nitrogen, produced only 1% of dry cell weight (DCW) in neutral lipids, while starch was rapidly accumulated up to 46% DCW. The increased in starch content was accompanied by a coordinated overexpression of genes shunting carbon towards starch synthesis, a response not seen in the oleaginous microalgae Nannochloropsis oceanica, Chlamydomonas reinhardtii or Chlorella vulgaris. Genes in the central carbon metabolism pathways, particularly those of the tricarboxylic acid cycle, were also simultaneously upregulated, indicating a robust interchange of carbon skeletons for anabolic and catabolic processes. In contrast, fatty acid and triacylglycerol synthesis genes were downregulated or unchanged, suggesting that lipids are not a preferred form of storage in these cells. This study reveals the transcriptomic influence behind storage reserve allocation in D. tertiolecta and provides valuable insights into the possible manipulation of genes for engineering microorganisms to synthesize products of interest.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/srep37235
dc.publisherNature Publishing Group
dc.relation.ispartofseries6;37235
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNitrogen depletion
dc.subjectDunaliella tertiolecta
dc.subjectStarch
dc.subjectFatty acid
dc.subjectTriacylglycerol
dc.subjectTricarboxylic acid cycle
dc.subjectMicroalgae
dc.subjectLipid production
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY & IMMUNOLOGY
dc.description.doi10.1038/srep37235
dc.description.sourcetitleScientific Reports
dc.description.volume6
dc.description.page1-13
dc.identifier.isiut000387961000001
dc.published.statePublished
dc.grant.idR-182-000-205-592
dc.grant.fundingagencyNational Research Foundation
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