Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13068-016-0671-2
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dc.titleThe dilemma for lipid productivity in green microalgae: importance of substrate provision in improving oil yield without sacrificing growth
dc.contributor.authorTan, Kenneth Wei Min
dc.contributor.authorLee, Yuan Kun
dc.date.accessioned2016-12-13T03:15:41Z
dc.date.available2016-12-13T03:15:41Z
dc.date.issued2016-11-22
dc.identifier.citationTan, Kenneth Wei Min, Lee, Yuan Kun (2016-11-22). The dilemma for lipid productivity in green microalgae: importance of substrate provision in improving oil yield without sacrificing growth. Biotechnology for Biofuels 9 (1) : 1-14. ScholarBank@NUS Repository. https://doi.org/10.1186/s13068-016-0671-2
dc.identifier.issn17546834
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132279
dc.description.abstractRising oil prices and concerns over climate change have resulted in more emphasis on research into renewable biofuels from microalgae. Unlike plants, green microalgae have higher biomass productivity, will not compete with food and agriculture, and do not require fertile land for cultivation. However, microalgae biofuels currently suffer from high capital and operating costs due to low yields and costly extraction methods. Microalgae grown under optimal conditions produce large amounts of biomass but with low neutral lipid content, while microalgae grown in nutrient starvation accumulate high levels of neutral lipids but are slow growing. Producing lipids while maintaining high growth rates is vital for biofuel production because high biomass productivity increases yield per harvest volume while high lipid content decreases the cost of extraction per unit product. Therefore, there is a need for metabolic engineering of microalgae to constitutively produce high amounts of lipids without sacrificing growth. Substrate availability is a rate-limiting step in balancing growth and fatty acid (FA) production because both biomass and FA synthesis pathways compete for the same substrates, namely acetyl-CoA and NADPH. In this review, we discuss the efforts made for improving biofuel production in plants and microorganisms, the challenges faced in achieving lipid productivity, and the important role of precursor supply for FA synthesis. The main focus is placed on the enzymes which catalyzed the reactions supplying acetyl-CoA and NADPH.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/s13068-016-0671-2
dc.publisherBioMed Central
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMicroalgae
dc.subjectLipid productivity
dc.subjectFatty acid
dc.subjectNitrogen depletion
dc.subjectAcetyl-CoA
dc.subjectNADPH
dc.subjectATP:citrate lyase
dc.subjectMalic enzyme
dc.subjectGlucose-6-phosphate dehydrogenase
dc.subjectPyruvate dehydrogenase
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY & IMMUNOLOGY
dc.description.doi10.1186/s13068-016-0671-2
dc.description.sourcetitleBiotechnology for Biofuels
dc.description.volume9
dc.description.issue1
dc.description.page1-14
dc.identifier.isiut000389379800001
dc.published.statePublished
dc.grant.idR-182-000-205-592
dc.grant.fundingagencyNational Research Foundation
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