Please use this identifier to cite or link to this item:
|dc.title||Systemic levels of cytokines and GAD-specific autoantibodies isotypes in Chinese IDDM patients|
|dc.identifier.citation||Ng, W.Y., Thai, A.C., Lui, K.F., Yeo, P.P.B., Cheah, J.S. (1999-02). Systemic levels of cytokines and GAD-specific autoantibodies isotypes in Chinese IDDM patients. Diabetes Research and Clinical Practice 43 (2) : 127-135. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-8227(98)00130-2|
|dc.description.abstract||It is not clear if a Th1/Th2 imbalance in Type 1 diabetes (insulin- dependent diabetes mellitus, IDDM) would lead to a particular antigen- specific IgG subclass dominant as had been shown in the mouse model. In new, onset Type 1 diabetics, an autoantibody response to glutamate decarboxylase (GADab) is frequently observed but the GADab subclass repertoire is not well- established. We determined the systemic levels of representative Th1 and Th2 cytokines and the GADab IgG subclass distribution in 41 Chinese IDDM patients of whom 26 were recently diagnosed (≤ 1 year) and 32 had GADab, to ascertain a likely association of antigen-specific antibody isotype and the Th1/Th2 dichotomy. With high-sensitivity ELISA systems that measure sub-picogram cytokine concentrations; 26 of the 41 patients (63.4%) had at least one of the pro-inflammatory Th1 cytokines (TNF-α, IFN-γ and IL-12) detected. Fewer patients (4/41) had the anti-inflammatory Th2 cytokine IL-4 detected. For IL- 10, all subjects had measurable quantities but only three diabetics had levels above the upper limit for healthy subjects (n = 20). Grouped according to the profile of detectable cytokines, there were 24 Th1, 2 Th2 and 2 Th0 patterns. GAD-specific IgG1 antibody was more frequently expressed; 22 of 32 GADab[+] patients. The rank order for the GADab subclasses was IgG1 > 4 > 3 > 2; IgG2 was found in 11 GADab[+] patients. Recent-onset diabetics have a similar ranking of the GAD-specific IgG subclasses. In human Type 1 diabetes, a predominance of GAD-specific IgG1 antibody response is observed together with a dominant Th1 cytokine pattern.|
|dc.description.sourcetitle||Diabetes Research and Clinical Practice|
|Appears in Collections:||Staff Publications|
Show simple item record
Files in This Item:
There are no files associated with this item.
checked on Aug 5, 2020
WEB OF SCIENCETM
checked on Aug 5, 2020
checked on Jul 31, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.