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Title: Immunohistochemical detection of Influenza virus infection in formalin-fixed tissues with anti-H5 monoclonal antibody recognizing FFWTILKP
Authors: He, F. 
Du, Q.
Ho, Y.
Kwang, J. 
Keywords: H5N1
IHC staining
Monoclonal antibody
Paraffin sections
Issue Date: Jan-2009
Citation: He, F., Du, Q., Ho, Y., Kwang, J. (2009-01). Immunohistochemical detection of Influenza virus infection in formalin-fixed tissues with anti-H5 monoclonal antibody recognizing FFWTILKP. Journal of Virological Methods 155 (1) : 25-33. ScholarBank@NUS Repository.
Abstract: The worldwide outbreak of avian influenza among poultry species and humans is associated with the H5N1 subtype of avian influenza A virus (AIV). This highlighted the need to develop safe H5 AIV diagnostic methods. 7H10, an H5-specific monoclonal antibody (Mab), can be used for immunohistochemical (IHC) staining for formalin-fixed tissue. An assortment of H5N1 tissue specimens infected naturally in paraffin sections from Asia, between years 2002-2006, including one human specimen, were tested. 7H10 detected H5 infection in all of these tissue samples infected naturally. In addition, 24 different human H5N1 isolates from Indonesia, 5 avian H5 isolates and 3 non-H5 isolates from Asia were inoculated into BALB/C mice and chicken embryos. Among these influenza viruses, 7H10 detected 28 of the 29 H5 virus strains by immunohistochemical staining, while none of non-H5 strains used in this study could be detected by 7H10, confirming its specificity to H5. Further, the eight-residue-long linear epitope, "FFWTILKP", identified through epitope mapping, enables 7H10 to detect >98.3% of H5 subtype viruses reported worldwide before 2007. This study describes a specific H5 diagnostic system with minimal possibility of exposure to live virus based on immunochemical staining. © 2008 Elsevier B.V. All rights reserved.
Source Title: Journal of Virological Methods
ISSN: 01660934
DOI: 10.1016/j.jviromet.2008.09.016
Appears in Collections:Staff Publications

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