Please use this identifier to cite or link to this item:
https://doi.org/10.1074/jbc.M202460200
DC Field | Value | |
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dc.title | Peritoneal CD5 + B-1 cells have signaling properties similar to tolerant B cells | |
dc.contributor.author | Wong, S.-C. | |
dc.contributor.author | Chew, W.-K. | |
dc.contributor.author | Tan, J.E.-L. | |
dc.contributor.author | Melendez, A.J. | |
dc.contributor.author | Francis, F. | |
dc.contributor.author | Lam, K.-P. | |
dc.date.accessioned | 2016-11-28T10:19:43Z | |
dc.date.available | 2016-11-28T10:19:43Z | |
dc.date.issued | 2002-08-23 | |
dc.identifier.citation | Wong, S.-C., Chew, W.-K., Tan, J.E.-L., Melendez, A.J., Francis, F., Lam, K.-P. (2002-08-23). Peritoneal CD5 + B-1 cells have signaling properties similar to tolerant B cells. Journal of Biological Chemistry 277 (34) : 30707-30715. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M202460200 | |
dc.identifier.issn | 00219258 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/131396 | |
dc.description.abstract | CD5 + B (or B-1) cells are the normal precursors of B cell chronic lymphocytic leukemia. They differ from conventional B (B-2) cells with respect to their phenotype and mitogenic responses and are often secretors of the natural polyreactive antibodies in the serum. The origin of B-1 cells remains controversial, and the relationship between B-1 cells and autoreactive B cells is unclear. Here, we compare the signaling pathways that are activated by the engagement of the B cell antigen receptor (BCR) in B-1 and B-2 cells. Stimulation of the BCR leads to the induced activation of the three major classes of mitogen-activated protein kinases (MAPKs), ERK, JNK, and p38 MAPK, as well as the Akt kinase and the transcription factors nuclear factor of activated T cells (NF-AT) and NF-κB in B-2 cells. In contrast, B-1 cells have constitutive activation of ERK and NF-AT but exhibit delayed JNK and lack p38 MAPK and NF-κB induction upon BCR cross-linking. The lack of NF-κB activation in B-1 cells may be due to a lack of Akt activation in these cells. Furthermore, our study using specific inhibitors reveals that the extended survival of B-1 cells in culture is not due to the constitutive activation of ERK; nor is it due to Akt signaling or Bcl-X L regulation, since these are not induced in B-1 cells. The current findings of altered MAPK and NF-AT activation and lack of NF-κB induction in B-1 cells indicate that these cells have signaling properties similar to tolerant B cells that are chronically exposed to self-antigens. Indeed, BCR stimulation of B-1 cells does not lead to their full activation as indicated by their lack of maximal up-regulation of specific markers such as CD25, CD69, and CD86. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M202460200 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.department | PAEDIATRICS | |
dc.description.doi | 10.1074/jbc.M202460200 | |
dc.description.sourcetitle | Journal of Biological Chemistry | |
dc.description.volume | 277 | |
dc.description.issue | 34 | |
dc.description.page | 30707-30715 | |
dc.description.coden | JBCHA | |
dc.identifier.isiut | 000177579800040 | |
Appears in Collections: | Staff Publications |
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