Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M202460200
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dc.titlePeritoneal CD5 + B-1 cells have signaling properties similar to tolerant B cells
dc.contributor.authorWong, S.-C.
dc.contributor.authorChew, W.-K.
dc.contributor.authorTan, J.E.-L.
dc.contributor.authorMelendez, A.J.
dc.contributor.authorFrancis, F.
dc.contributor.authorLam, K.-P.
dc.date.accessioned2016-11-28T10:19:43Z
dc.date.available2016-11-28T10:19:43Z
dc.date.issued2002-08-23
dc.identifier.citationWong, S.-C., Chew, W.-K., Tan, J.E.-L., Melendez, A.J., Francis, F., Lam, K.-P. (2002-08-23). Peritoneal CD5 + B-1 cells have signaling properties similar to tolerant B cells. Journal of Biological Chemistry 277 (34) : 30707-30715. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M202460200
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131396
dc.description.abstractCD5 + B (or B-1) cells are the normal precursors of B cell chronic lymphocytic leukemia. They differ from conventional B (B-2) cells with respect to their phenotype and mitogenic responses and are often secretors of the natural polyreactive antibodies in the serum. The origin of B-1 cells remains controversial, and the relationship between B-1 cells and autoreactive B cells is unclear. Here, we compare the signaling pathways that are activated by the engagement of the B cell antigen receptor (BCR) in B-1 and B-2 cells. Stimulation of the BCR leads to the induced activation of the three major classes of mitogen-activated protein kinases (MAPKs), ERK, JNK, and p38 MAPK, as well as the Akt kinase and the transcription factors nuclear factor of activated T cells (NF-AT) and NF-κB in B-2 cells. In contrast, B-1 cells have constitutive activation of ERK and NF-AT but exhibit delayed JNK and lack p38 MAPK and NF-κB induction upon BCR cross-linking. The lack of NF-κB activation in B-1 cells may be due to a lack of Akt activation in these cells. Furthermore, our study using specific inhibitors reveals that the extended survival of B-1 cells in culture is not due to the constitutive activation of ERK; nor is it due to Akt signaling or Bcl-X L regulation, since these are not induced in B-1 cells. The current findings of altered MAPK and NF-AT activation and lack of NF-κB induction in B-1 cells indicate that these cells have signaling properties similar to tolerant B cells that are chronically exposed to self-antigens. Indeed, BCR stimulation of B-1 cells does not lead to their full activation as indicated by their lack of maximal up-regulation of specific markers such as CD25, CD69, and CD86.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M202460200
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentPAEDIATRICS
dc.description.doi10.1074/jbc.M202460200
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume277
dc.description.issue34
dc.description.page30707-30715
dc.description.codenJBCHA
dc.identifier.isiut000177579800040
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