Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/131312
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dc.titleIntracytoplasmic sperm injection of mouse oocytes with 5 mM Ca2+ at different intervals
dc.contributor.authorAhmadi, A.A.
dc.contributor.authorNg, S.C.
dc.contributor.authorLiow, S.L.
dc.contributor.authorAli, J.
dc.contributor.authorBongso, A.
dc.contributor.authorRatnam, S.S.
dc.date.accessioned2016-11-28T10:18:44Z
dc.date.available2016-11-28T10:18:44Z
dc.date.issued1995
dc.identifier.citationAhmadi, A.A., Ng, S.C., Liow, S.L., Ali, J., Bongso, A., Ratnam, S.S. (1995). Intracytoplasmic sperm injection of mouse oocytes with 5 mM Ca2+ at different intervals. Human Reproduction 10 (2) : 431-435. ScholarBank@NUS Repository.
dc.identifier.issn02681161
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131312
dc.description.abstractThe objective of this investigation was to determine whether intracytoplasmic sperm injection (ICSI) can be performed in the mouse. Metaphase II oocytes were obtained from F1 hybrid mice (C57BL x CBA) by i.p. injections of 10 IU pregnant mare's serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) administered 48 h apart. Oocytes with cumulus oophorus were retrieved 13-14 h post HCG. Cumulus was dispersed with 0.1% hyaluronidase. Mouse spermatozoa were obtained from the cauda epididymides of males of the same strain. The spermatozoa were processed by the standard swim-up procedure. The harvested spermatozoa were then incubated for 1.5 h to allow capacitation. Healthy oocytes were injected with 3-4 pl 5 mM Ca2+, followed by one live morphologically normal spermatozoon into the cytoplasm at intervals of 0, 0.5, 1, 2 and 3 h. The proportion of 2-cell embryos that developed from oocytes injected with Ca2+ and spermatozoa ranged between 29.5 and 36.5% in all groups, with no statistical difference between treatments. Chromosomal analysis showed that two-thirds of the ICSI-derived 2-cell embryos were diploid. The proportion of pathenogenetically activated embryos in the ICSI groups was similar to that in the control group (8-10%) which was injected with Ca2+ and polyvinyl pyrrolidone only. The proportion of blastocysts that developed in culture from the ICSI-derived 2-cell embryos was of the order of 36-42%. Some blastocysts were used for cell number counts. There was a significant increase in total and inner cell mass counts of blastocysts in which the spermatozoon was injected at 2 and 3 h following Ca2+. The remaining blastocysts were transferred to day 3 pseudopregnant mice, of which 33% subsequently became pregnant. Of the blastocysts transferred, 16-25% developed to term in vivo. No deformities were observed in the pups. We believe this is the first report of live-birth following mouse ICSI.
dc.sourceScopus
dc.subjectCa2+ injection
dc.subjectIntracytoplasmic sperm injection
dc.subjectMicro-injection
dc.subjectMouse oocyte
dc.subjectSpermatozoa
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.sourcetitleHuman Reproduction
dc.description.volume10
dc.description.issue2
dc.description.page431-435
dc.description.codenHUREE
dc.identifier.isiutNOT_IN_WOS
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