Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.0405881101
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dc.titlePhenotypic conversion of human mammary carcinoma cells by autocrine human growth hormone
dc.contributor.authorMukhina, S.
dc.contributor.authorMertani, H.C.
dc.contributor.authorGuo, K.
dc.contributor.authorLee, K.-O.
dc.contributor.authorGluckman, P.D.
dc.contributor.authorLobie, P.E.
dc.date.accessioned2016-11-28T10:17:37Z
dc.date.available2016-11-28T10:17:37Z
dc.date.issued2004-10-19
dc.identifier.citationMukhina, S., Mertani, H.C., Guo, K., Lee, K.-O., Gluckman, P.D., Lobie, P.E. (2004-10-19). Phenotypic conversion of human mammary carcinoma cells by autocrine human growth hormone. Proceedings of the National Academy of Sciences of the United States of America 101 (42) : 15166-15171. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.0405881101
dc.identifier.issn00278424
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131220
dc.description.abstractWe report here that autocrine production of human growth hormone (hGH) results in a phenotypic conversion of mammary carcinoma cells such that they exhibit the morphological and molecular characteristics of a mesenchymal cell, including expression of fibronectin and vimentin. Autocrine production of hGH resulted in reduced plakoglobin expression and relocalization of E-cadherin to the cytoplasm, leading to dissolution of cell-cell contacts and decreased cell height. These phenotypic changes were accompanied by an increase in cell motility, elevated activity of specific matrix metalloproteinases, and an acquired ability to invade a reconstituted basement membrane. Forced expression of plakoglobin significantly decreased mammary carcinoma cell migration and invasion stimulated by autocrine hGH. In vivo, autocrine hGH stimulated local invasion of mammary carcinoma cells concomitant with a prominent stromal reaction in comparison with well delineated and capsulated growth of mammary carcinoma cells lacking autocrine production of hGH. Thus, autocrine production of hGH by mammary carcinoma cells is sufficient for generation of an invasive phenotype. Therapeutic targeting of autocrine hGH may provide a mechanistic approach to prevent metastatic extension of human mammary carcinoma.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1073/pnas.0405881101
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.description.doi10.1073/pnas.0405881101
dc.description.sourcetitleProceedings of the National Academy of Sciences of the United States of America
dc.description.volume101
dc.description.issue42
dc.description.page15166-15171
dc.description.codenPNASA
dc.identifier.isiut000224688700035
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