Please use this identifier to cite or link to this item: https://doi.org/10.1007/s004360000365
DC FieldValue
dc.titleExposure of Blastocystis species to a cytotoxic monoclonal antibody
dc.contributor.authorTan, K.S.
dc.contributor.authorIbrahim, M.
dc.contributor.authorNg, G.
dc.contributor.authorNasirudeen, A.
dc.contributor.authorHo, L.
dc.contributor.authorYap, E.
dc.contributor.authorSingh, M.
dc.date.accessioned2016-11-28T10:14:38Z
dc.date.available2016-11-28T10:14:38Z
dc.date.issued2001
dc.identifier.citationTan, K.S., Ibrahim, M., Ng, G., Nasirudeen, A., Ho, L., Yap, E., Singh, M. (2001). Exposure of Blastocystis species to a cytotoxic monoclonal antibody. Parasitology Research 87 (7) : 534-538. ScholarBank@NUS Repository. https://doi.org/10.1007/s004360000365
dc.identifier.issn09320113
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/130958
dc.description.abstractA recently described cytotoxic monoclonal antibody (mAb 1D5) raised against Blastocystis hominis isolate B, was tested for reactivity with 13 different isolates of Blastocystis. The isolates used were previously isolated from humans, rats and reptiles and were maintained as axenised cultures throughout the course of this stady. Five B. hominis isolates (B, C, E, G and H) were found to react with mAb 1D5 in immunoblotting studies and the indirect fluorescence antibody test. The pattern of fluorescence observed for all five isolates was diffuse and patchy. Immunoblotting studies revealed that mAb 1D5 reacted with a 29-30-kDa protein found in all five isolates. Results of a cytotoxic assay showed that the mAb exhibited a complement-independent cytotoxic effect on all the exposed isolates. Microscopic observations showed differences in morphology between the Blastocystis cells exposed and unexposed to mAb. Acridine orange staining performed on both exposed and unexposed cells showed similar internal structures when viewed under fluorescence microscopy.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s004360000365
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1007/s004360000365
dc.description.sourcetitleParasitology Research
dc.description.volume87
dc.description.issue7
dc.description.page534-538
dc.description.codenPARRE
dc.identifier.isiut000169598400005
Appears in Collections:Staff Publications

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