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Title: Detection and genetic characterisation of qnrB in hospital isolates of Klebsiella pneumoniae in Singapore
Authors: Teo, J.W.P.
Ng, K.Y.
Lin, R.T.P. 
Keywords: Plasmid
Quinolone resistance
Issue Date: Feb-2009
Citation: Teo, J.W.P., Ng, K.Y., Lin, R.T.P. (2009-02). Detection and genetic characterisation of qnrB in hospital isolates of Klebsiella pneumoniae in Singapore. International Journal of Antimicrobial Agents 33 (2) : 177-180. ScholarBank@NUS Repository.
Abstract: Polymerase chain reaction (PCR) screening of 116 ciprofloxacin-resistant Klebsiella pneumoniae hospital isolates for the presence of qnr genes that mediate plasmid quinolone resistance revealed that none were positive for qnrA or qnrS. However, qnrB was detected in ca. 5.2% of the isolates. Southern hybridisation demonstrated that the qnrB-hybridising plasmids were large (>70 kb) and capable of transferring quinolone resistance by conjugation. Sequence analysis of the qnrB genes detected in this study showed that they were identical to previously identified qnrB1, qnrB4 and qnrB6 genes, although a novel variant designated qnrB20 was also identified. Analysis of the genetic environment around the cloned qnrB genes showed that they were present in diverse plasmid backbones, sometimes within novel genetic contexts, but always associated with mobile or transposable elements. © 2008 Elsevier B.V. and the International Society of Chemotherapy.
Source Title: International Journal of Antimicrobial Agents
ISSN: 09248579
DOI: 10.1016/j.ijantimicag.2008.08.019
Appears in Collections:Staff Publications

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