Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0012153
DC Field | Value | |
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dc.title | Cdc42 interaction with N-WASP and Toca-1 regulates membrane tubulation, vesicle formation and vesicle motility: Implications for endocytosis | |
dc.contributor.author | Bu, W. | |
dc.contributor.author | Lim, K.B. | |
dc.contributor.author | Yu, Y.H. | |
dc.contributor.author | Chou, A.M. | |
dc.contributor.author | Sudhaharan, T. | |
dc.contributor.author | Ahmed, S. | |
dc.date.accessioned | 2016-10-22T07:45:11Z | |
dc.date.available | 2016-10-22T07:45:11Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Bu, W., Lim, K.B., Yu, Y.H., Chou, A.M., Sudhaharan, T., Ahmed, S. (2010). Cdc42 interaction with N-WASP and Toca-1 regulates membrane tubulation, vesicle formation and vesicle motility: Implications for endocytosis. PLoS ONE 5 (8) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0012153 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/128863 | |
dc.description.abstract | Transducer of Cdc42-dependent actin assembly (Toca-1) consists of an F-BAR domain, a Cdc42 binding site and an SH3 domain. Toca-1 interacts with N-WASP, an activator of actin nucleation that binds Cdc42. Cdc42 may play an important role in regulating Toca-1 and N-WASP functions. We report here that the cellular expression of Toca-1 and N-WASP induces membrane tubulation and the formation of motile vesicles. Marker and uptake analysis suggests that the tubules and vesicles are associated with clathrin-mediated endocytosis. Forster resonance energy transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) analysis shows that Cdc42, N-WASP and Toca-1 form a trimer complex on the membrane tubules and vesicles and that Cdc42 interaction with N-WASP is critical for complex formation. Modulation of Cdc42 interaction with Toca-1 and/or N-WASP affects membrane tubulation, vesicle formation and vesicle motility. Thus Cdc42 may influence endocytic membrane trafficking by regulating the formation and activity of the Toca-1/N-WASP complex. © 2010 Bu et al. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0012153 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE | |
dc.description.doi | 10.1371/journal.pone.0012153 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 5 | |
dc.description.issue | 8 | |
dc.description.page | - | |
dc.identifier.isiut | 000280862200012 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications |
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10_1371_journal_pone_0012153.pdf | 7.88 MB | Adobe PDF | OPEN | Published | View/Download |
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