Please use this identifier to cite or link to this item: https://doi.org/10.1002/glia.22476
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dc.titleNeuronal activity promotes myelination via a cAMP pathway
dc.contributor.authorMalone, M.
dc.contributor.authorGary, D.
dc.contributor.authorYang, I.H.
dc.contributor.authorMiglioretti, A.
dc.contributor.authorHoudayer, T.
dc.contributor.authorThakor, N.
dc.contributor.authorMcdonald, J.
dc.date.accessioned2016-10-19T08:44:55Z
dc.date.available2016-10-19T08:44:55Z
dc.date.issued2013-06
dc.identifier.citationMalone, M., Gary, D., Yang, I.H., Miglioretti, A., Houdayer, T., Thakor, N., Mcdonald, J. (2013-06). Neuronal activity promotes myelination via a cAMP pathway. GLIA 61 (6) : 843-854. ScholarBank@NUS Repository. https://doi.org/10.1002/glia.22476
dc.identifier.issn08941491
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128766
dc.description.abstractNeuronal activity promotes myelination in vivo and in vitro. However, the molecular events that mediate activity-dependent myelination are not completely understood. Seven, daily 1 h sessions of patterned electrical stimulation (ESTIM) promoted myelin segment formation in mixed cultures of dorsal root ganglion (DRG) neurons and oligodendrocytes (OLs); the increase in myelination was frequency-dependent. Myelin segment formation was also enhanced following exposure of DRGs to ESTIM prior to OL addition, suggesting that ESTIM promotes myelination in a manner involving neuron-specific signaling. Cyclic adenosine monophosphate (cAMP) levels in DRGs were increased three-fold following ESTIM, and artificially increasing cAMP mimicked the ability of ESTIM to promote myelination. Alternatively, inhibiting the cAMP pathway suppressed ESTIM-induced myelination. We used compartmentalized, microfluidic platforms to isolate DRG soma from OLs and assessed cell-type specific effects of ESTIM on myelination. A selective increase or decrease in DRG cAMP levels resulted in enhanced or suppressed myelination, respectively. This work describes a novel role for the cAMP pathway in neurons that results in enhanced myelination. © 2013 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/glia.22476
dc.sourceScopus
dc.subjectElectrical stimulation
dc.subjectFrequency
dc.subjectMicrofluidic chamber
dc.subjectMyelin
dc.subjectOligodendrocyte
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.contributor.departmentELECTRICAL ENGINEERING
dc.description.doi10.1002/glia.22476
dc.description.sourcetitleGLIA
dc.description.volume61
dc.description.issue6
dc.description.page843-854
dc.description.codenGLIAE
dc.identifier.isiut000317992500002
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