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https://doi.org/10.1038/nmeth.1668
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dc.title | Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function | |
dc.contributor.author | Zhao, S. | |
dc.contributor.author | Ting, J.T. | |
dc.contributor.author | Atallah, H.E. | |
dc.contributor.author | Qiu, L. | |
dc.contributor.author | Tan, J. | |
dc.contributor.author | Gloss, B. | |
dc.contributor.author | Augustine, G.J. | |
dc.contributor.author | Deisseroth, K. | |
dc.contributor.author | Luo, M. | |
dc.contributor.author | Graybiel, A.M. | |
dc.contributor.author | Feng, G. | |
dc.date.accessioned | 2016-10-19T08:44:11Z | |
dc.date.available | 2016-10-19T08:44:11Z | |
dc.date.issued | 2011-09 | |
dc.identifier.citation | Zhao, S., Ting, J.T., Atallah, H.E., Qiu, L., Tan, J., Gloss, B., Augustine, G.J., Deisseroth, K., Luo, M., Graybiel, A.M., Feng, G. (2011-09). Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nature Methods 8 (9) : 745-755. ScholarBank@NUS Repository. https://doi.org/10.1038/nmeth.1668 | |
dc.identifier.issn | 15487091 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/128698 | |
dc.description.abstract | Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type-specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type-specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior. © 2011 Nature America, Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/nmeth.1668 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1038/nmeth.1668 | |
dc.description.sourcetitle | Nature Methods | |
dc.description.volume | 8 | |
dc.description.issue | 9 | |
dc.description.page | 745-755 | |
dc.identifier.isiut | 000294439100011 | |
Appears in Collections: | Staff Publications |
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