Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M111.288357
DC FieldValue
dc.titleFibroblast growth factor-23-mediated inhibition of renal phosphate transport in mice requires sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) and synergizes with parathyroid hormone
dc.contributor.authorWeinman, E.J.
dc.contributor.authorSteplock, D.
dc.contributor.authorShenolikar, S.
dc.contributor.authorBiswas, R.
dc.date.accessioned2016-10-19T08:43:10Z
dc.date.available2016-10-19T08:43:10Z
dc.date.issued2011-10-28
dc.identifier.citationWeinman, E.J., Steplock, D., Shenolikar, S., Biswas, R. (2011-10-28). Fibroblast growth factor-23-mediated inhibition of renal phosphate transport in mice requires sodium-hydrogen exchanger regulatory factor-1 (NHERF-1) and synergizes with parathyroid hormone. Journal of Biological Chemistry 286 (43) : 37216-37221. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M111.288357
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128612
dc.description.abstractFibroblast growth factor-23 (FGF-23) inhibits sodium-dependent phosphate transport in brush border membrane vesicles derived from hormone-treated kidney slices of the mouse and in mouse proximal tubule cells by processes involving mitogen-activated protein kinase (MAPK) but not protein kinase A (PKA) or protein kinase C (PKC). By contrast, phosphate transport in brush border membrane vesicles and proximal tubule cells from sodium-hydrogen exchanger regulatory factor-1 (NHERF-1)-null mice were resistant to the inhibitory effect of FGF-23 (10 -9 M). Infection of NHERF-1-null proximal tubule cells with wild-type adenovirus-GFP-NHERF-1 increased basal phosphate transport and restored the inhibitory effect of FGF-23. Infection with adenovirus-GFP-NHERF-1 containing a S77A or T95D mutation also increased basal phosphate transport, but the cells remained resistant to FGF-23 (10 -9 M). Low concentrations of FGF-23 (10 -13 M) and PTH (10 -11 M) individually did not inhibit phosphate transport or activate PKA, PKC, or MAPK. When combined, however, these hormones markedly inhibited phosphate transport associated with activation of PKC and PKA but not MAPK. These studies indicate that FGF-23 inhibits phosphate transport in the mouse kidney by processes that involve the scaffold protein NHERF-1. In addition, FGF-23 synergizes with PTH to inhibit phosphate transport by facilitating the activation of the PTH signal transduction pathway.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M111.288357
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1074/jbc.M111.288357
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume286
dc.description.issue43
dc.description.page37216-37221
dc.description.codenJBCHA
dc.identifier.isiut000296542400022
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.