Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M109.094359
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dc.titleSodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney
dc.contributor.authorWeinman, E.J.
dc.contributor.authorBiswas, R.
dc.contributor.authorSteplock, D.
dc.contributor.authorDouglass, T.S.
dc.contributor.authorCunninghamand, R.
dc.contributor.authorShenolikar, S.
dc.date.accessioned2016-10-19T08:43:05Z
dc.date.available2016-10-19T08:43:05Z
dc.date.issued2010-04-30
dc.identifier.citationWeinman, E.J., Biswas, R., Steplock, D., Douglass, T.S., Cunninghamand, R., Shenolikar, S. (2010-04-30). Sodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney. Journal of Biological Chemistry 285 (18) : 13454-13460. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M109.094359
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128605
dc.description.abstractDopamine inhibited phosphate transport in isolated renal brush border membrane vesicles and in cultured renal proximal tubule cells from wild-type but not from NHERF-1 null mice. Co-immunoprecipitation experiments established that NHERF-1 associated with D1-like receptors. In wild-type mice, dopamine stimulated cAMP accumulation and protein kinase C (PKC) activity in renal proximal tubule cells, an effect that was abolished by SCH-23390, a D1-like receptor antagonist. In NHERF-1 null kidney tissue; however, dopamine failed to stimulate either cAMP accumulation or PKC activity. Infection of proximal tubule cells from NHERF-1 null mice with adenovirus-green fluorescent protein-NHERF-1 restored the ability of dopamine to stimulate cAMP and PKC. Finally, in 32P-labeled wild-type proximal tubule cells and in opossum kidney cells, dopamine increased NHERF-1 phosphorylation at serine 77 of the PDZ I domain of NHERF-1, a site previously shown to attenuate binding of cellular targets including the Npt2a (sodium-dependent phosphate transporter 2a). Together, these studies establish that NHERF-1 plays a key role in dopamine signaling and is also a downstream target of D1-like receptors in the mouse kidney. These studies suggest a novel role for the PDZ adapter protein NHERF-1 in coordinating dopamine signals that inhibit renal phosphate transport.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M109.094359
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1074/jbc.M109.094359
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume285
dc.description.issue18
dc.description.page13454-13460
dc.description.codenJBCHA
dc.identifier.isiut000276987700017
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