Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2012.01.091
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dc.titleChromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts
dc.contributor.authorWatanabe, T.M.
dc.contributor.authorHiguchi, S.
dc.contributor.authorKawauchi, K.
dc.contributor.authorTsukasaki, Y.
dc.contributor.authorIchimura, T.
dc.contributor.authorFujita, H.
dc.date.accessioned2016-10-18T06:27:14Z
dc.date.available2016-10-18T06:27:14Z
dc.date.issued2012-02-24
dc.identifier.citationWatanabe, T.M., Higuchi, S., Kawauchi, K., Tsukasaki, Y., Ichimura, T., Fujita, H. (2012-02-24). Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts. Biochemical and Biophysical Research Communications 418 (4) : 742-747. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2012.01.091
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128517
dc.description.abstractSkeletal muscle undergoes complicated differentiation steps that include cell-cycle arrest, cell fusion, and maturation, which are controlled through sequential expression of transcription factors. During muscle differentiation, remodeling of the epigenetic landscape is also known to take place on a large scale, determining cell fate. In an attempt to determine the extent of epigenetic remodeling during muscle differentiation, we characterized the plasticity of the chromatin structure using C2C12 myoblasts. Differentiation of C2C12 cells was induced by lowering the serum concentration after they had reached full confluence, resulting in the formation of multi-nucleated myotubes. Upon induction of differentiation, the nucleus size decreased whereas the aspect ratio increased, indicating the presence of force on the nucleus during differentiation. Movement of the nucleus was also suppressed when differentiation was induced, indicating that the plasticity of chromatin changed upon differentiation. To evaluate the histone dynamics during differentiation, FRAP experiment was performed, which showed an increase in the immobile fraction of histone proteins when differentiation was induced. To further evaluate the change in the histone dynamics during differentiation, FCS was performed, which showed a decrease in histone mobility on differentiation. We here show that the plasticity of chromatin decreases upon differentiation, which takes place in a stepwise manner, and that it can be used as an index for the differentiation stage during myogenesis using the state diagram developed with the parameters obtained in this study. © 2012 Elsevier Inc..
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2012.01.091
dc.sourceScopus
dc.subjectEpigenetics
dc.subjectHistone
dc.subjectImaging
dc.subjectMyogenesis
dc.subjectNucleus
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.1016/j.bbrc.2012.01.091
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume418
dc.description.issue4
dc.description.page742-747
dc.description.codenBBRCA
dc.identifier.isiut000301332100027
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