Please use this identifier to cite or link to this item: https://doi.org/10.1039/c4an00317a
DC FieldValue
dc.titleApoptotic pathways of U937 leukemic monocytes investigated by infrared microspectroscopy and flow cytometry
dc.contributor.authorBirarda, G.
dc.contributor.authorBedolla, D.E.
dc.contributor.authorMitri, E.
dc.contributor.authorPacor, S.
dc.contributor.authorGrenci, G.
dc.contributor.authorVaccari, L.
dc.date.accessioned2016-10-18T06:27:11Z
dc.date.available2016-10-18T06:27:11Z
dc.date.issued2014-06-21
dc.identifier.citationBirarda, G., Bedolla, D.E., Mitri, E., Pacor, S., Grenci, G., Vaccari, L. (2014-06-21). Apoptotic pathways of U937 leukemic monocytes investigated by infrared microspectroscopy and flow cytometry. Analyst 139 (12) : 3097-3106. ScholarBank@NUS Repository. https://doi.org/10.1039/c4an00317a
dc.identifier.issn13645528
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128512
dc.description.abstractApoptosis is a strictly regulated cell death mechanism that plays a pivotal role in the normal evolution of multicellular organisms. Its misregulation has been associated with many diseases, making its early and reliable detection a key point for modern cellular biology. In this paper, we propose the use of infrared microspectroscopy (IRMS) as a label-free methodology for the detection of apoptotic-related biochemical processes induced on U937 leukemic monocytes by serum starvation and CCCP-exposure. The spectroscopic results are in agreement with parallel Flow Cytometry (FC) experiments, where plasma membrane integrity and mitochondrial activity were assessed. Spectroscopic outcomes complement FC data and allow drawing a more complete picture of the apoptotic pathways. In particular, we established that the two apoptosis-inducing treatments, cell starvation and CCCP exposure, affect the cell cycle in a different way. With the former, cell death is preceded by a cell cycle arrest, whereas the latter causes an increased cell cycle progression. Spectral data demonstrate that for both conditions apoptosis proceeds through the accumulation of lipid droplets within cells. Moreover, we were able to establish a spectral marker for DNA condensation/fragmentation: the enhancement of the PhI band component centred at ∼1206 cm-1, which is more sensitive than the relative intensity of the PhII band to which phospholipids and carbohydrates also contribute significantly. In conclusion, we demonstrate that the intrinsic multi-parametric nature of IRMS and its application on cells under physiological conditions can be well exploited for the investigation of apoptotic pathways. © the Partner Organisations 2014.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1039/c4an00317a
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.1039/c4an00317a
dc.description.sourcetitleAnalyst
dc.description.volume139
dc.description.issue12
dc.description.page3097-3106
dc.description.codenANALA
dc.identifier.isiut000336737900022
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

19
checked on Oct 23, 2020

WEB OF SCIENCETM
Citations

19
checked on Oct 23, 2020

Page view(s)

46
checked on Oct 17, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.