Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.24756
DC FieldValue
dc.titleCovalently grafted BMP-7 peptide to reduce macrophage/monocyte activity: An in vitro study on cobalt chromium alloy
dc.contributor.authorTan, H.C.
dc.contributor.authorPoh, C.K.
dc.contributor.authorCAI YANLI
dc.contributor.authorSoe, M.T.
dc.contributor.authorWang, W.
dc.date.accessioned2016-09-07T05:36:29Z
dc.date.available2016-09-07T05:36:29Z
dc.date.issued2013-03
dc.identifier.citationTan, H.C., Poh, C.K., CAI YANLI, Soe, M.T., Wang, W. (2013-03). Covalently grafted BMP-7 peptide to reduce macrophage/monocyte activity: An in vitro study on cobalt chromium alloy. Biotechnology and Bioengineering 110 (3) : 969-979. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.24756
dc.identifier.issn00063592
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/127080
dc.description.abstractCobalt chromium (CoCr) alloy is widely used in orthopedic implants but its functional longevity is susceptible to inflammation related complications. Reduction of the development of chronic inflammation on the biomaterial surface would enhance direct bone-implant bonding and improve implant survival and long-term results. The BMP-7 peptide was derived from the knuckle epitope of bone morphogenic protein-7 (BMP-7) and was conjugated via a cysteine amino acid at the N-terminus. Mouse RAW 264.7 monocytes/macrophages were seeded on the CoCr substrates and inflammation was induced via lipopolysaccharide (LPS) challenge. The effects of BMP-7 peptide on inflammation were evaluated by measuring the expression of inflammatory markers like toll-like receptor-4 (TLR-4), tumor necrosis factor-α (TNF-α), and monocyte chemotactic protein-1 (MCP-1). ELISA and qPCR assays were used to study the inflammatory signals. BMP-7 signaling pathway activation was shown by the presence of phosphorylation of Smad1/5/8. Utilizing the reactivity of polydopamine films to immobilize BMP-7 peptide onto metal substrates may provide a promising approach for applications in situations where reduction of inflammation around implants would be beneficial in improving surgical outcome, bone healing, and implant integration. © 2012 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/bit.24756
dc.sourceScopus
dc.subjectBioengineering
dc.subjectBone morphogenic protein
dc.subjectCovalent binding
dc.subjectImplant
dc.subjectInflammation
dc.subjectSurface modification
dc.typeArticle
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.description.doi10.1002/bit.24756
dc.description.sourcetitleBiotechnology and Bioengineering
dc.description.volume110
dc.description.issue3
dc.description.page969-979
dc.description.codenBIBIA
dc.identifier.isiut000313806400030
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

7
checked on Oct 16, 2019

WEB OF SCIENCETM
Citations

8
checked on Oct 8, 2019

Page view(s)

26
checked on Oct 11, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.