Please use this identifier to cite or link to this item: https://doi.org/10.1093/hmg/dds061
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dc.titleCdk1, but not Cdk2, is the sole Cdk that is essential and sufficient to drive resumption of meiosis in mouse oocytes
dc.contributor.authorAdhikari, D.
dc.contributor.authorZheng, W.
dc.contributor.authorShen, Y.
dc.contributor.authorGorre, N.
dc.contributor.authorNing, Y.
dc.contributor.authorHalet, G.
dc.contributor.authorKaldis, P.
dc.contributor.authorLiu, K.
dc.date.accessioned2016-09-07T03:09:48Z
dc.date.available2016-09-07T03:09:48Z
dc.date.issued2012-06
dc.identifier.citationAdhikari, D., Zheng, W., Shen, Y., Gorre, N., Ning, Y., Halet, G., Kaldis, P., Liu, K. (2012-06). Cdk1, but not Cdk2, is the sole Cdk that is essential and sufficient to drive resumption of meiosis in mouse oocytes. Human Molecular Genetics 21 (11) : 2476-2484. ScholarBank@NUS Repository. https://doi.org/10.1093/hmg/dds061
dc.identifier.issn09646906
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/126976
dc.description.abstractMammalian oocytes are arrested at the prophase of meiosis I during fetal or postnatal development, and the meiosis is resumed by the preovulatory surge of luteinizing hormone. The in vivo functional roles of cyclin-dependent kinases (Cdks) during the resumption of meiosis in mammalian oocytes are largely unknown. Previous studies have shown that deletions of Cdk3, Cdk4 or Cdk6 in mice result in viable animals with normal oocyte maturation, indicating that these Cdks are not essential for the meiotic maturation of oocytes. In addition, conventional knockout of Cdk1 and Cdk2 leads to embryonic lethality and postnatal follicular depletion, respectively, making it impossible to study the functions of Cdk1 and Cdk2 in oocyte meiosis. In this study, we generated conditional knockout mice with oocyte-specific deletions of Cdk1 and Cdk2. We showed that the lack of Cdk1, but not of Cdk2, leads to female infertility due to a failure of the resumption of meiosis in the oocyte. Re-introduction of Cdk1 mRNA into Cdk1-null oocytes largely resumed meiosis. Thus, Cdk1 is the sole Cdk that is essential and sufficient to drive resumption of meiosis in mouse oocytes. We also found that Cdk1 maintains the phosphorylation status of protein phosphatase 1 and lamin A/C in oocytes in order for meiosis resumption to occur. © The Author 2012. Published by Oxford University Press. All rights reserved.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1093/hmg/dds061
dc.description.sourcetitleHuman Molecular Genetics
dc.description.volume21
dc.description.issue11
dc.description.page2476-2484
dc.description.codenHMGEE
dc.identifier.isiut000304053100008
Appears in Collections:Staff Publications

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